UniProt Accession

 AIF1_RAT; P55009; P70491 

Genbank Protein ID

 U17919; D82069; AB000818; BX883046 

Genbank Nucleotide ID

 AAA80105.1; BAA11533.1; BAA19189.1; CAE83999.1 

Protein Name

 Allograft inflammatory factor 1 

Protein Synonyms/Alias

 AIF-1; Ionized calcium-binding adapter molecule 1; MRF-1; Microglia response factor 

Gene Name

 Aif1 

Gene Synonyms/Alias

 Iba1; Mrf1 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
11	SKDLQGGKAFGLLKA	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play an role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation (By similarity). 

Sequence Annotation

 DOMAIN 45 80 EF-hand 1.
 DOMAIN 81 115 EF-hand 2; degenerate.
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 11 11 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Actin-binding; Calcium; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Membrane; Metal-binding; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 147 AA 

Protein Sequence

Gene Ontology

 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0030027; C:lamellipodium; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0043204; C:perikaryon; IDA:RGD.
 GO:0001891; C:phagocytic cup; ISS:UniProtKB.
 GO:0032587; C:ruffle membrane; IEA:UniProtKB-SubCell.
 GO:0051015; F:actin filament binding; ISS:UniProtKB.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0051017; P:actin filament bundle assembly; ISS:UniProtKB.
 GO:0030041; P:actin filament polymerization; ISS:UniProtKB.
 GO:0031668; P:cellular response to extracellular stimulus; IEP:RGD.
 GO:0032870; P:cellular response to hormone stimulus; IEP:RGD.
 GO:0071346; P:cellular response to interferon-gamma; IEP:UniProtKB.
 GO:0006954; P:inflammatory response; IEP:UniProtKB.
 GO:0042116; P:macrophage activation; NAS:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; IDA:RGD.
 GO:0010629; P:negative regulation of gene expression; IMP:RGD.
 GO:0006911; P:phagocytosis, engulfment; ISS:UniProtKB.
 GO:1900087; P:positive regulation of G1/S transition of mitotic cell cycle; ISS:UniProtKB.
 GO:0090026; P:positive regulation of monocyte chemotaxis; ISS:UniProtKB.
 GO:0014739; P:positive regulation of muscle hyperplasia; IMP:RGD.
 GO:0045429; P:positive regulation of nitric oxide biosynthetic process; IDA:RGD.
 GO:0001934; P:positive regulation of protein phosphorylation; IMP:RGD.
 GO:0071673; P:positive regulation of smooth muscle cell chemotaxis; ISS:UniProtKB.
 GO:0048661; P:positive regulation of smooth muscle cell proliferation; ISS:UniProtKB.
 GO:2000406; P:positive regulation of T cell migration; ISS:UniProtKB.
 GO:0042102; P:positive regulation of T cell proliferation; ISS:UniProtKB.
 GO:0016601; P:Rac protein signal transduction; ISS:UniProtKB.
 GO:0048678; P:response to axon injury; IEP:RGD.
 GO:0051602; P:response to electrical stimulus; IEP:RGD.
 GO:0097178; P:ruffle assembly; ISS:UniProtKB. 

Interpro

 IPR011992; EF-hand-like_dom.
 IPR002048; EF_hand_dom. 

Pfam

  

SMART

  

PROSITE

 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 

PRINTS