| Tag | Content |
|---|
| CPLM ID | CPLM-008700 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | mRNA-decapping enzyme subunit 2 |
Protein Synonyms/Alias | Protein PSU1 |
Gene Name | DCP2 |
Gene Synonyms/Alias | PSU1; YNL118C; N1917 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 596 | SVEWGPGKSSPSTQS | ubiquitination | [1] | | 604 | SSPSTQSKQNSSVGM | ubiquitination | [1] | | 614 | SSVGMQNKYRQEIHI | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Catalytic component of the decapping complex necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Decapping is the major pathway of mRNA degradation in yeast. It occurs through deadenylation, decapping and subsequent 5' to 3' exonucleolytic decay of the transcript body. |
Sequence Annotation | DOMAIN 101 228 Nudix hydrolase.
MOTIF 134 155 Nudix box.
METAL 149 149 Manganese (By similarity).
METAL 153 153 Manganese (By similarity).
MOD_RES 116 116 Phosphoserine.
MOD_RES 439 439 Phosphoserine.
MOD_RES 677 677 Phosphothreonine.
MOD_RES 679 679 Phosphoserine.
MOD_RES 682 682 Phosphoserine.
MOD_RES 751 751 Phosphoserine.
MOD_RES 771 771 Phosphoserine.
MOD_RES 773 773 Phosphoserine.
MOD_RES 778 778 Phosphoserine. |
Keyword | 3D-structure; Complete proteome; Cytoplasm; Hydrolase; Manganese; Metal-binding; mRNA processing; Nonsense-mediated mRNA decay; Phosphoprotein; Reference proteome; RNA-binding. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 970 AA |
Protein Sequence | MSLPLRHALE NVTSVDRILE DLLVRFIINC PNEDLSSVER ELFHFEEASW FYTDFIKLMN 60
PTLPSLKIKS FAQLIIKLCP LVWKWDIRVD EALQQFSKYK KSIPVRGAAI FNENLSKILL 120
VQGTESDSWS FPRGKISKDE NDIDCCIREV KEEIGFDLTD YIDDNQFIER NIQGKNYKIF 180
LISGVSEVFN FKPQVRNEID KIEWFDFKKI SKTMYKSNIK YYLINSMMRP LSMWLRHQRQ 240
IKNEDQLKSY AEEQLKLLLG ITKEEQIDPG RELLNMLHTA VQANSNNNAV SNGQVPSSQE 300
LQHLKEQSGE HNQQKDQQSS FSSQQQPSIF PSLSEPFANN KNVIPPTMPM ANVFMSNPQL 360
FATMNGQPFA PFPFMLPLTN NSNSANPIPT PVPPNFNAPP NPMAFGVPNM HNLSGPAVSQ 420
PFSLPPAPLP RDSGYSSSSP GQLLDILNSK KPDSNVQSSK KPKLKILQRG TDLNSIKQNN 480
NDETAHSNSQ ALLDLLKKPT SSQKIHASKP DTSFLPNDSV SGIQDAEYED FESSSDEEVE 540
TARDERNSLN VDIGVNVMPS EKDSRRSQKE KPRNDASKTN LNASAESNSV EWGPGKSSPS 600
TQSKQNSSVG MQNKYRQEIH IGDSDAYEVF ESSSDEEDGK KLEELEQTQD NSKLISQDIL 660
KENNFQDGEV PHRDMPTESN KSINETVGLS STTNTVKKVP KVKILKRGET FASLANDKKA 720
FDSSSNVSSS KDLLQMLRNP ISSTVSSNQQ SPKSQHLSGD EEIMMMLKRN SVSKPQNSEE 780
NASTSSINDA NASELLGMLK QKEKDITAPK QPYNVDSYSQ KNSAKGLLNI LKKNDSTGYP 840
RTEGGPSSEM STSMKRNDAT NNQELDKNST ELLNYLKPKP LNDGYENISN KDSSHELLNI 900
LHGNKNSSAF NNNVYATDGY SLASDNNENS SNKLLNMLQN RSSAINEPNF DVRSNGTSGS 960
NELLSILHRK 970 |
Gene Ontology | GO:0000932; C:cytoplasmic mRNA processing body; IDA:SGD. GO:0005634; C:nucleus; IDA:SGD. GO:0050072; F:m7G(5')pppN diphosphatase activity; IDA:SGD. GO:0030145; F:manganese ion binding; IEA:InterPro. GO:0003729; F:mRNA binding; IPI:SGD. GO:0000290; P:deadenylation-dependent decapping of nuclear-transcribed mRNA; IDA:SGD. GO:0031087; P:deadenylation-independent decapping of nuclear-transcribed mRNA; IMP:SGD. GO:0006397; P:mRNA processing; IEA:UniProtKB-KW. GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:UniProtKB-KW. GO:0034063; P:stress granule assembly; IMP:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |