CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013460
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kelch-like protein 26 
Protein Synonyms/Alias
  
Gene Name
 KLHL26 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
32RPNSTADKNGALKCTubiquitination[1]
37ADKNGALKCTFSAPSubiquitination[1]
78REAFPAHKVVLAACSubiquitination[1]
107SQDVIELKGVSARGLubiquitination[1, 2, 3]
329SDRSVSSKVYQLPEPubiquitination[1, 2, 3, 4, 5, 6, 7]
447WGYACSLKRRTWGHAubiquitination[1, 4, 5, 7]
475YGISVEDKKALHCYDubiquitination[1]
476GISVEDKKALHCYDPubiquitination[1]
491VADQWEFKAPMSEPRubiquitination[1, 4, 6, 7]
560GCCLLERKIYIVGGYubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
 DOMAIN 63 130 BTB.
 DOMAIN 165 266 BACK.
 REPEAT 310 361 Kelch 1.
 REPEAT 362 413 Kelch 2.
 REPEAT 414 460 Kelch 3.
 REPEAT 461 508 Kelch 4.
 REPEAT 510 559 Kelch 5.
 REPEAT 561 608 Kelch 6.
 MOD_RES 2 2 N-acetylalanine.  
Keyword
 Acetylation; Complete proteome; Kelch repeat; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 615 AA 
Protein Sequence
MAESGGSSGG AGGGGAFGAG PGPERPNSTA DKNGALKCTF SAPSHSTSLL QGLATLRAQG 60
QLLDVVLTIN REAFPAHKVV LAACSDYFRA MFTGGMREAS QDVIELKGVS ARGLRHIIDF 120
AYSAEVTLDL DCVQDVLGAA VFLQMLPVVE LCEEFLKAAM SVETCLNIGQ MATTFSLASL 180
RESVDAFTFR HFLQIAEEED FLRLPLERLV FFLQSNRLQS CAEIDLFRAA VRWLQHDPAR 240
RPRASHVLCH IRFPLMQSSE LVDSVQTLDI MVEDVLCRQY LLEAFNYQVL PFRQHEMQSP 300
RTAVRSDVPS LVTFGGTPYT DSDRSVSSKV YQLPEPGARH FRELTEMEVG CSHTCVAVLD 360
NFVYVAGGQH LQYRSGEGAV DACYRYDPHL NRWLRLQAMQ ESRIQFQLNV LCGMVYATGG 420
RNRAGSLASV ERYCPRRNEW GYACSLKRRT WGHAGAASGG RLYISGGYGI SVEDKKALHC 480
YDPVADQWEF KAPMSEPRVL HAMVGAGGRI YALGGRMDHV DRCFDVLAVE YYVPETDQWT 540
SVSPMRAGQS EAGCCLLERK IYIVGGYNWR LNNVTGIVQV YNTDTDEWER DLHFPESFAG 600
IACAPVLLPR AGTRR 615 
Gene Ontology
  
Interpro
 IPR011705; BACK.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR015916; Gal_Oxidase_b-propeller.
 IPR017096; Kelch-like_gigaxonin.
 IPR006652; Kelch_1. 
Pfam
 PF07707; BACK
 PF00651; BTB
 PF01344; Kelch_1 
SMART
 SM00875; BACK
 SM00225; BTB
 SM00612; Kelch 
PROSITE
 PS50097; BTB 
PRINTS