UniProt Accession

 NFIP1_MOUSE; Q8R0W6; Q9EQH8 

Genbank Protein ID

 AF220209; AK050560; BC026372 

Genbank Nucleotide ID

 AAG44248.1; BAC34324.1; AAH26372.1 

Protein Name

 NEDD4 family-interacting protein 1 

Protein Synonyms/Alias

 NEDD4 WW domain-binding protein 5 

Gene Name

 Ndfip1 

Gene Synonyms/Alias

 N4wbp5 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
83	YDEAERTKTEATIPL	ubiquitination	[1]
204	INYAKVRKMPETFSN	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Activates HECT domain-containing several E3 ubiquitin- protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation. In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity (By similarity). Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1- to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate (By similarity). 

Sequence Annotation

 MOTIF 40 42 PY 1.
 MOTIF 65 67 PY 2.
 MOTIF 74 76 PY 3.  

Keyword

 Complete proteome; Endosome; Golgi apparatus; Membrane; Reference proteome; Repeat; Secreted; Transmembrane; Transmembrane helix; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 221 AA 

Protein Sequence

Gene Ontology

 GO:0005938; C:cell cortex; IDA:MGI.
 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
 GO:0030173; C:integral to Golgi membrane; IC:MGI.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0004871; F:signal transducer activity; IEA:Compara.
 GO:0006879; P:cellular iron ion homeostasis; IEA:Compara.
 GO:0010629; P:negative regulation of gene expression; IEA:Compara.
 GO:0050728; P:negative regulation of inflammatory response; IMP:MGI.
 GO:0032713; P:negative regulation of interleukin-4 production; IMP:MGI.
 GO:0048294; P:negative regulation of isotype switching to IgE isotypes; IMP:MGI.
 GO:0051224; P:negative regulation of protein transport; IEA:Compara.
 GO:0042130; P:negative regulation of T cell proliferation; IMP:MGI.
 GO:0032410; P:negative regulation of transporter activity; IEA:Compara.
 GO:0002829; P:negative regulation of type 2 immune response; IMP:MGI.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; IEA:Compara.
 GO:0045732; P:positive regulation of protein catabolic process; IDA:MGI.
 GO:0031398; P:positive regulation of protein ubiquitination; IEA:Compara.
 GO:0048302; P:regulation of isotype switching to IgG isotypes; IMP:MGI.
 GO:0045619; P:regulation of lymphocyte differentiation; IMP:MGI.
 GO:0002761; P:regulation of myeloid leukocyte differentiation; IMP:MGI.
 GO:0043162; P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IGI:MGI. 

Interpro

 IPR019325; NEDD4/BSD2. 

Pfam

 PF10176; DUF2370 

SMART

  

PROSITE

  

PRINTS