UniProt Accession

 KHDR1_MOUSE; Q60749; A2ACH3; B2KG38; Q3U8T3; Q60735; Q7M4N5; Q99M33 

Genbank Protein ID

 U17961; U17046; AK050520; AK152084; AL669834; CU210913; BC002051 

Genbank Nucleotide ID

 AAA86693.1; AAA64997.1; BAC34303.1; BAE30934.1; CAM13766.1; CAQ51789.1; AAH02051.1 

Protein Name

 KH domain-containing, RNA-binding, signal transduction-associated protein 1 

Protein Synonyms/Alias

 GAP-associated tyrosine phosphoprotein p62; Src-associated in mitosis 68 kDa protein; Sam68; p21 Ras GTPase-activating protein-associated p62; p68 

Gene Name

 Khdrbs1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
21	SSGRSCSKDPSGAHP	acetylation	[1]
96	PSATAAVKMEPENKY	acetylation	[2]
102	VKMEPENKYLPELMA	acetylation	[2]
102	VKMEPENKYLPELMA	ubiquitination	[3]
152	YLDLFSHKNMKLKER	ubiquitination	[3]
165	ERVLIPVKQYPKFNF	ubiquitination	[3]
169	IPVKQYPKFNFVGKI	ubiquitination	[3]
175	PKFNFVGKILGPQGN	acetylation	[2, 4]
175	PKFNFVGKILGPQGN	ubiquitination	[3]
185	GPQGNTIKRLQEETG	ubiquitination	[3]
194	LQEETGAKISVLGKG	ubiquitination	[3]
432	APPARPVKGAYREHP	ubiquitination	[3]

Reference

 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 

Functional Description

 Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain- containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. Positively regulates the association of constitutive transport element (CTE)-containing mRNA with large polyribosomes and translation initiation. May not be involved in the nucleocytoplasmic export of unspliced (CTE)- containing RNA species. 

Sequence Annotation

 DOMAIN 171 197 KH.
 MOD_RES 20 20 Phosphoserine (By similarity).
 MOD_RES 29 29 Phosphoserine (By similarity).
 MOD_RES 45 45 Asymmetric dimethylarginine; alternate
 MOD_RES 45 45 Omega-N-methylated arginine; by PRMT1;
 MOD_RES 52 52 Asymmetric dimethylarginine; alternate
 MOD_RES 52 52 Omega-N-methylated arginine; by PRMT1;
 MOD_RES 58 58 Phosphoserine (By similarity).
 MOD_RES 113 113 Phosphoserine.
 MOD_RES 175 175 N6-acetyllysine (By similarity).
 MOD_RES 291 291 Omega-N-methylated arginine; by PRMT1 (By
 MOD_RES 304 304 Asymmetric dimethylarginine; alternate
 MOD_RES 304 304 Omega-N-methylated arginine; by PRMT1;
 MOD_RES 310 310 Omega-N-methylarginine; alternate (By
 MOD_RES 310 310 Omega-N-methylated arginine; by PRMT1;
 MOD_RES 315 315 Omega-N-methylarginine; alternate (By
 MOD_RES 315 315 Omega-N-methylated arginine; by PRMT1;
 MOD_RES 320 320 Dimethylated arginine; alternate (By
 MOD_RES 320 320 Omega-N-methylarginine; alternate (By
 MOD_RES 320 320 Omega-N-methylated arginine; by PRMT1;
 MOD_RES 325 325 Omega-N-methylarginine; alternate (By
 MOD_RES 325 325 Omega-N-methylated arginine; by PRMT1;
 MOD_RES 331 331 Dimethylated arginine; alternate (By
 MOD_RES 331 331 Omega-N-methylated arginine; by PRMT1;
 MOD_RES 340 340 Omega-N-methylarginine; alternate (By
 MOD_RES 340 340 Omega-N-methylated arginine; by PRMT1;
 MOD_RES 346 346 Omega-N-methylated arginine; by PRMT1 (By
 MOD_RES 435 435 Phosphotyrosine; by PTK6 (By similarity).
 MOD_RES 440 440 Phosphotyrosine; by PTK6 (By similarity).
 MOD_RES 443 443 Phosphotyrosine; by PTK6 (By similarity).  

Keyword

 Acetylation; Cell cycle; Complete proteome; Direct protein sequencing; Membrane; Methylation; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; SH3-binding; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 443 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0070618; C:Grb2-Sos complex; IEA:Compara.
 GO:0016020; C:membrane; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0003723; F:RNA binding; IDA:UniProtKB.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0007166; P:cell surface receptor signaling pathway; IDA:UniProtKB.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:MGI.
 GO:0046833; P:positive regulation of RNA export from nucleus; ISS:UniProtKB.
 GO:0045948; P:positive regulation of translational initiation; ISS:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR004087; KH_dom.
 IPR004088; KH_dom_type_1. 

Pfam

 PF00013; KH_1 

SMART

 SM00322; KH 

PROSITE

 PS50084; KH_TYPE_1 

PRINTS