CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004623
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Translation initiation factor IF-2 
Protein Synonyms/Alias
  
Gene Name
 infB 
Gene Synonyms/Alias
 BSU16630 
Created Date
 July 27, 2013 
Organism
 Bacillus subtilis (strain 168) 
NCBI Taxa ID
 224308 
Lysine Modification
Position
Peptide
Type
References
266YQIEENGKKITFLDTacetylation[1]
601RLHRIIYKVIDEIEAacetylation[1]
Reference
 [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis.
 Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG.
 Proteomics. 2013 May;13(10-11):1726-36. [PMID: 23468065] 
Functional Description
 One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex. 
Sequence Annotation
 NP_BIND 226 233 GTP (By similarity).
 NP_BIND 272 276 GTP (By similarity).
 NP_BIND 326 329 GTP (By similarity).
 REGION 220 368 G-domain.  
Keyword
 Alternative initiation; Complete proteome; Cytoplasm; GTP-binding; Initiation factor; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 716 AA 
Protein Sequence
MAKMRVYEYA KALNVSSKEI LTALKNMDLE VNNHMAMLEE KAIKKLDAKY KKGGARAKSQ 60
KPAETNKNKQ PQGVNQQSAG NQPNKIRDGK KNDVQNNQFN KNKKNNNNKK NKRNNNNNKN 120
QHQQKPVKPK KELPEKITFS GTLTVGALAE ELGKEPSELI KKLMLLGVMA TINQELDKDT 180
IELIASEYGV ETEEVIVLEE TELEKYEEPD NEEDLEIRPP VVTIMGHVDH GKTTLLDSIR 240
KTKVVEGEAG GITQHIGAYQ IEENGKKITF LDTPGHAAFT TMRARGAEVT DITILVVAAD 300
DGVMPQTVEA INHAKAAEVP IIVAVNKIDK ESANPDRVMQ ELTEYGLVPE AWGGETIFVP 360
LSALTGKGID ELVEMILLVS EVEELKANPN RQAKGTVIEA ELDKGRGSVA TLLVQTGTLH 420
VGDPIVVGNT FGRVRAMVND IGRRVKTAGP STPVEITGLN DVPQAGDQFL VFKDEKTARS 480
VGEARASKQL EEQRSDKAKL SLDDLFEQIK QGDVKDINLI VKADVQGSAE ALTAALQKIE 540
VEGVKVKIIH TGVGAITESD IILASASNAI VIGFNVRPDG NAKSTAEAEN VDIRLHRIIY 600
KVIDEIEAAM KGMLDPEYEE KVIGQVEVRQ TFKVSKIGTI AGGYVTEGTI TRDSGLRLIR 660
DGVVIFEGEV DVLKRFKDDV KEVSQGYECG ITIKKYNDIR EGDILEAFVM QEIERT 716 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:HAMAP.
 GO:0003743; F:translation initiation factor activity; IEA:HAMAP.
 GO:0006184; P:GTP catabolic process; IEA:GOC. 
Interpro
 IPR000795; EF_GTP-bd_dom.
 IPR006847; IF2_N.
 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR000178; TF_IF2_bacterial-like.
 IPR015760; TIF_IF2.
 IPR023115; TIF_IF2_dom3.
 IPR004161; Transl_elong_EFTu/EF1A_2.
 IPR009000; Transl_elong_init/rib_B-barrel. 
Pfam
 PF00009; GTP_EFTU
 PF03144; GTP_EFTU_D2
 PF11987; IF-2
 PF04760; IF2_N 
SMART
  
PROSITE
 PS01176; IF2 
PRINTS