CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023418
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein ush 
Protein Synonyms/Alias
 Protein U-shaped 
Gene Name
 ush 
Gene Synonyms/Alias
 CG2762 
Created Date
 July 27, 2013 
Organism
 Drosophila melanogaster (Fruit fly) 
NCBI Taxa ID
 7227 
Lysine Modification
Position
Peptide
Type
References
25TVDSRDSKDLSAQDIacetylation[1]
168LASDPALKPDAKELTacetylation[1]
354DIRFNNIKTYRAHKQacetylation[1]
1138VKTYLAHKQFYCKNKacetylation[1]
Reference
 [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation.
 Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C.
 Sci Signal. 2011 Jul 26;4(183):ra48. [PMID: 21791702] 
Functional Description
 Transcription regulator that modulates expression mediated by transcription factors of the GATA family such as pnr and srp. Represses transcription of proneural achaete-scute complex (AS-C), which is usually activated by pnr. Involved in cardiogenesis, blood, and eye development. During hematopoiesis, it is required to restrict the number of crystal cells, probably via its interaction with the isoform SrpNC of srp. Negatively regulates expression of sr. Probably acts by interacting with the GATA-type zinc finger of proteins such as pnr and srp, possibly antagonizing the interaction between the GATA-type zinc finger and some cofactor. 
Sequence Annotation
 ZN_FING 208 231 C2HC-type 1.
 ZN_FING 279 301 C2H2-type 1.
 ZN_FING 341 362 C2HC-type 2.
 ZN_FING 726 749 C2HC-type 3.
 ZN_FING 797 820 C2HC-type 4.
 ZN_FING 882 907 C2H2-type 2.
 ZN_FING 910 932 C2H2-type 3.
 ZN_FING 983 1006 C2H2-type 4.
 ZN_FING 1119 1142 C2HC-type 5.
 MOD_RES 116 116 Phosphoserine.
 MOD_RES 118 118 Phosphoserine.
 MOD_RES 1013 1013 Phosphoserine.
 MOD_RES 1015 1015 Phosphoserine.
 MOD_RES 1017 1017 Phosphoserine.
 MOD_RES 1156 1156 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1191 AA 
Protein Sequence
MLSSNTRGDC SDTAEEMTVD SRDSKDLSAQ DIGEQKQQQM EDQLEDQLND SRDPQNNNNN 60
IDDDADEDAE FEEPEKANPQ QDQDLGETEM EQEHDLQQED LQQELPANSP STPPRSPSSP 120
QLIPKLEQPA TPPSEPEASP CPSPSPCPTP KYPKVRLNAL LASDPALKPD AKELTLPDSR 180
LLAPPPLVKP DTQAQPEVAE PLLKPARFMC LPCGIAFSSP STLEAHQAYY CSHRIKDTDE 240
AGSDKSGAGG SGATAGDAAG LTGGSTEPPA KMARTGKQYG CTQCSYSADK KVSLNRHMRM 300
HQTSPAAPTL AGLPSLLQNG IAPPGVTPNP MEDSSSQQTD RYCSHCDIRF NNIKTYRAHK 360
QHYCSSRRPE GQLTPKPDAS PGAGSGPGSA GGSIGVSAQA ATPGKLSPQA RNKTPTPAMV 420
AVAAAAAAAA ASLQATPHSH PPFLALPTHP IIIVPCSLIR AASFIPGPLP TPNSGIVNPE 480
TTCFTVDNGT IKPLATALVG ATLEPERPSA PSSAAEATEA KSSPPEPKRK EAGLTRESAP 540
LDLSLRRSPI TLNSLSLRQR QLRNALLDVE EVLLAGVGTG KENVETPRGG GSVTPEQIVC 600
APSLPSSPSM SPSPKRRAIS PRSSGAGSAS SMSPPGLNVA VPHLLDMRSM LPADFGLSES 660
LLAKTNPELA LKLAAAAAAA AVAGSSGAAA FPPASLPAQT SSGNPGSGGS AGGAQQPQIY 720
VKKGVSKCME CNIVFCKYEN YLAHKQHYCS ARSQEGASEV DVKSAVSPSI AGAGGLGAGA 780
AEAASSVETT PVAYQQLICA ACGIKYTSLD NLRAHQNYYC PKGGAVAAPA ATPTDPGQLG 840
MPKEKCGKCK TLHEIGLPCP PPVANPLAAP TVNPQPATNS LNKCPVCGVV SPTAALAKKH 900
MEMHGTVKAY RCSICQYKGN TLRGMRTHIR THFDKKTSDV NEELYMTCIF EEDASALSQE 960
LVTPTGASTT TGHDSMDHPS QMFNCDYCNY VSTYKGNVLR HMKLMHPHVA INSPSISPDT 1020
RDQDVTSNPT TNQHSNSDVS NGEAPSFHIK SEPLDPPPTV NLVHENNNSP IATPHIKAEP 1080
IEVGADAAPG GLVPPMTSPL GNSSSVAAAA AAAAEVMKKY CSTCDISFNY VKTYLAHKQF 1140
YCKNKPIRPE ASDSPSPNHL GGGVAVGLGI GGLVGGHGQQ KNKENLQEAA I 1191 
Gene Ontology
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0008134; F:transcription factor binding; IMP:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0046665; P:amnioserosa maintenance; IMP:FlyBase.
 GO:0022416; P:chaeta development; IGI:FlyBase.
 GO:0048749; P:compound eye development; IMP:FlyBase.
 GO:0007393; P:dorsal closure, leading edge cell fate determination; NAS:FlyBase.
 GO:0007398; P:ectoderm development; TAS:FlyBase.
 GO:0007390; P:germ-band shortening; IMP:FlyBase.
 GO:0008258; P:head involution; NAS:FlyBase.
 GO:0007507; P:heart development; IMP:FlyBase.
 GO:0035167; P:larval lymph gland hemopoiesis; IMP:FlyBase.
 GO:0009996; P:negative regulation of cell fate specification; TAS:FlyBase.
 GO:0042690; P:negative regulation of crystal cell differentiation; TAS:FlyBase.
 GO:0043433; P:negative regulation of sequence-specific DNA binding transcription factor activity; IDA:FlyBase.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:FlyBase.
 GO:0042440; P:pigment metabolic process; TAS:FlyBase.
 GO:0002805; P:regulation of antimicrobial peptide biosynthetic process; IMP:FlyBase.
 GO:0007362; P:terminal region determination; IGI:FlyBase.
 GO:0008293; P:torso signaling pathway; IGI:FlyBase.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like. 
Pfam
  
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS