CPLM-006923

Genbank Nucleotide ID

RNA polymerase II-associated protein 1

Protein Synonyms/Alias

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Position	Peptide	Type	References
77	KFPGLLNKLDSKLLY	acetylation	[1]
114	LTKTDISKVTFLRRT	acetylation	[1]
167	RVEGTFNKTDKWQHP	acetylation	[1]
170	GTFNKTDKWQHPVKK	acetylation	[1]

[1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]

Functional Description

The PAF1 complex is a multifunctional complex. Involved in transcription initiation via genetic interactions with TATA- binding proteins. Involved in elongation. It regulates 3'-end formation of snR47 by modulating the recruitment or stable association of NRD1 and NAB3 with RNA polymerase II. Also has a role in transcription-coupled histone modification. Required for activation of the RAD6/UBC2-BRE1 ubiquitin ligase complex, which ubiquitinates histone H2B to form H2BK123ub1. Also required for the methylation of histone H3 by the COMPASS complex to form H3K4me, by SET2 to form H3K36me, and by DOT1 to form H3K79me. RNA polymerase II associated protein important for transcription of a subset of genes. Required for both positive and negative regulation. Negatively regulates MAK16 expression. Also required for efficient CLN2 transcription in late G1 and may be involved in transcription of galactose-inducible genes.

MOD_RES 147 147 Phosphoserine.
MOD_RES 422 422 Phosphothreonine.

Activator; Complete proteome; Direct protein sequencing; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0016593; C:Cdc73/Paf1 complex; IPI:SGD.
GO:0035327; C:transcriptionally active chromatin; IDA:SGD.
GO:0003682; F:chromatin binding; IDA:SGD.
GO:0000983; F:RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity; IDA:SGD.
GO:0001089; F:TFIIF-class binding transcription factor activity; IMP:SGD.
GO:0000183; P:chromatin silencing at rDNA; IMP:SGD.
GO:0070911; P:global genome nucleotide-excision repair; IMP:SGD.
GO:0031124; P:mRNA 3'-end processing; IMP:SGD.
GO:0045910; P:negative regulation of DNA recombination; IMP:SGD.
GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:SGD.
GO:2001255; P:positive regulation of histone H3-K36 trimethylation; IMP:SGD.
GO:2001165; P:positive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues; IMP:SGD.
GO:2001209; P:positive regulation of transcription elongation from RNA polymerase I promoter; IDA:SGD.
GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IMP:SGD.
GO:0031938; P:regulation of chromatin silencing at telomere; IMP:SGD.
GO:2001173; P:regulation of histone H2B conserved C-terminal lysine ubiquitination; IDA:SGD.
GO:0051569; P:regulation of histone H3-K4 methylation; IMP:SGD.
GO:0034401; P:regulation of transcription by chromatin organization; IMP:SGD.
GO:0000114; P:regulation of transcription involved in G1 phase of mitotic cell cycle; IMP:SGD.
GO:0090262; P:regulation of transcription-coupled nucleotide-excision repair; IGI:SGD.
GO:0006364; P:rRNA processing; IMP:SGD.
GO:0031126; P:snoRNA 3'-end processing; IMP:SGD.
GO:0001015; P:snoRNA transcription from an RNA polymerase II promoter; IMP:SGD.
GO:0006362; P:transcription elongation from RNA polymerase I promoter; IMP:SGD.
GO:0006368; P:transcription elongation from RNA polymerase II promoter; IMP:SGD.

IPR007133; RNA_pol_II-assoc_Paf1.