CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000631
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitochondrial import inner membrane translocase subunit TIM44 
Protein Synonyms/Alias
  
Gene Name
 Timm44 
Gene Synonyms/Alias
 Mimt44; Tim44 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
146SDLGRKIKEGVEEAAacetylation[1]
177LGKTAAFKAISQGVEacetylation[2, 3]
177LGKTAAFKAISQGVEsuccinylation[3]
217RTEFAGAKFKESKVFacetylation[1, 3]
217RTEFAGAKFKESKVFsuccinylation[3]
415VYDGDPDKVQRMLYVacetylation[4]
Reference
 [1] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 
Functional Description
 Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source. 
Sequence Annotation
 NP_BIND 166 173 ATP (Potential).
 MOD_RES 180 180 Phosphoserine (By similarity).  
Keyword
 ATP-binding; Complete proteome; Membrane; Mitochondrion; Mitochondrion inner membrane; Nucleotide-binding; Phosphoprotein; Protein transport; Reference proteome; Transit peptide; Translocation; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 452 AA 
Protein Sequence
MAAAALRGGW CRCPRRCLGS GIQFLSSHNL PHGSSYQISR PGRELTLTKS YSSGSRKGFL 60
SGLLDNIKQE LAKNKEMKES IKKFRDEAKK LEESDALQEA RRKYKSIESE TVRTSEAIKK 120
KLGELTGTVK ESLDEVSKSD LGRKIKEGVE EAARTAKQSA ESVSKSGEKL GKTAAFKAIS 180
QGVESVKKEL DESVLGQTGP YRRPERLRKR TEFAGAKFKE SKVFEANEEA LGVVLHKDSK 240
WYQQWKDFKD NNVVFNRFFE MKMKYDESDN VLIRASRALT DKVTDLLGGL FSKTEMSEVL 300
TEILRVDPTF DKDHFLHQCE TDIIPNILEA MISGELDILK DWCYEATYSQ LAHPIQQAKA 360
LGFQFHSRIL DISNVDLAMG KMMEQGPVLI VTFQAQVVMV IKNSKGEVYD GDPDKVQRML 420
YVWALCRDQE ELNPYAAWRL LDISASSTEQ IL 452 
Gene Ontology
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0005759; C:mitochondrial matrix; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0015450; F:P-P-bond-hydrolysis-driven protein transmembrane transporter activity; IEA:InterPro.
 GO:0006886; P:intracellular protein transport; IEA:InterPro. 
Interpro
 IPR017303; Tim44.
 IPR007379; Tim44-like_dom. 
Pfam
 PF04280; Tim44 
SMART
 SM00978; Tim44 
PROSITE
  
PRINTS