CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005785
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 60S ribosomal protein L8-B 
Protein Synonyms/Alias
 L4; L4-1; RP6; YL5 
Gene Name
 RPL8B 
Gene Synonyms/Alias
 KRB1; RPL4B; YLL045C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
15APAPFGAKSTKSNKAmethylation[1]
23STKSNKAKNPLTHSTacetylation[2]
43IGQAVQPKRNLSRYVmethylation[1]
111EKKERLTKEAAAIAEacetylation[3]
111EKKERLTKEAAAIAEubiquitination[4]
120AAAIAEGKSKQDASPubiquitination[4]
146VVSLIENKKAKLVLIacetylation[3]
181GVPYAIIKGKARLGTacetylation[3]
241GGGILGNKAQAKMDKmethylation[1]
Reference
 [1] Identification of arginine- and lysine-methylation in the proteome of Saccharomyces cerevisiae and its functional implications.
 Pang CN, Gasteiger E, Wilkins MR.
 BMC Genomics. 2010 Feb 5;11:92. [PMID: 20137074]
 [2] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [4] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Cytoplasm; Direct protein sequencing; Reference proteome; Ribonucleoprotein; Ribosomal protein. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 256 AA 
Protein Sequence
MAPGKKVAPA PFGAKSTKSN KAKNPLTHST PKNFGIGQAV QPKRNLSRYV KWPEYVRLQR 60
QKKILSIRLK VPPTIAQFQY TLDRNTAAET FKLFNKYRPE TAAEKKERLT KEAAAIAEGK 120
SKQDASPKPY AVKYGLNHVV SLIENKKAKL VLIANDVDPI ELVVFLPALC KKMGVPYAII 180
KGKARLGTLV NQKTSAVAAL TEVRAEDEAA LAKLVSTIDA NFADKYDEVK KHWGGGILGN 240
KAQAKMDKRA KTSDSA 256 
Gene Ontology
 GO:0022625; C:cytosolic large ribosomal subunit; TAS:SGD.
 GO:0003735; F:structural constituent of ribosome; TAS:SGD.
 GO:0002181; P:cytoplasmic translation; TAS:SGD.
 GO:0042254; P:ribosome biogenesis; IEA:InterPro. 
Interpro
 IPR001921; Ribosomal_L7A/L8.
 IPR004038; Ribosomal_L7Ae/L30e/S12e/Gad45.
 IPR018492; Ribosomal_L7Ae/L8/Nhp2.
 IPR004037; Ribosomal_L7Ae_CS. 
Pfam
 PF01248; Ribosomal_L7Ae 
SMART
  
PROSITE
 PS01082; RIBOSOMAL_L7AE 
PRINTS
 PR00881; L7ARS6FAMILY.
 PR00882; RIBOSOMALL7A.