CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023627
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 F-box/WD repeat-containing protein 1A 
Protein Synonyms/Alias
 E3RSIkappaB; Epididymis tissue protein Li 2a; F-box and WD repeats protein beta-TrCP; pIkappaBalpha-E3 receptor subunit 
Gene Name
 BTRC 
Gene Synonyms/Alias
 BTRCP; FBW1A; FBXW1A 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
11AEAVLQEKALKFMCSubiquitination[1]
14VLQEKALKFMCSMPRubiquitination[1]
73PRKIIPEKNSLRQTYubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys- 21' and 'Lys-22'. SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1/nuclear factor NF-kappa-B p105 subunit, ATF4, SMAD3, SMAD4, CDC25A, DLG1, FBXO5 and probably NFKB2. SCF(BTRC) mediates the ubiquitination of phosphorylated SNAI1. May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3. 
Sequence Annotation
 DOMAIN 190 228 F-box.
 REPEAT 301 338 WD 1.
 REPEAT 341 378 WD 2.
 REPEAT 381 418 WD 3.
 REPEAT 424 461 WD 4.
 REPEAT 464 503 WD 5.
 REPEAT 505 541 WD 6.
 REPEAT 553 590 WD 7.
 REGION 128 177 Homodimerization domain D.
 REGION 190 228 Required for down-regulation of SNAI1.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Host-virus interaction; Ligase; Nucleus; Polymorphism; Reference proteome; Repeat; Ubl conjugation pathway; WD repeat; Wnt signaling pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 605 AA 
Protein Sequence
MDPAEAVLQE KALKFMCSMP RSLWLGCSSL ADSMPSLRCL YNPGTGALTA FQNSSEREDC 60
NNGEPPRKII PEKNSLRQTY NSCARLCLNQ ETVCLASTAM KTENCVAKTK LANGTSSMIV 120
PKQRKLSASY EKEKELCVKY FEQWSESDQV EFVEHLISQM CHYQHGHINS YLKPMLQRDF 180
ITALPARGLD HIAENILSYL DAKSLCAAEL VCKEWYRVTS DGMLWKKLIE RMVRTDSLWR 240
GLAERRGWGQ YLFKNKPPDG NAPPNSFYRA LYPKIIQDIE TIESNWRCGR HSLQRIHCRS 300
ETSKGVYCLQ YDDQKIVSGL RDNTIKIWDK NTLECKRILT GHTGSVLCLQ YDERVIITGS 360
SDSTVRVWDV NTGEMLNTLI HHCEAVLHLR FNNGMMVTCS KDRSIAVWDM ASPTDITLRR 420
VLVGHRAAVN VVDFDDKYIV SASGDRTIKV WNTSTCEFVR TLNGHKRGIA CLQYRDRLVV 480
SGSSDNTIRL WDIECGACLR VLEGHEELVR CIRFDNKRIV SGAYDGKIKV WDLVAALDPR 540
APAGTLCLRT LVEHSGRVFR LQFDEFQIVS SSHDDTILIW DFLNDPAAQA EPPRSPSRTY 600
TYISR 605 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0019005; C:SCF ubiquitin ligase complex; IDA:UniProtKB.
 GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
 GO:0031145; P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; TAS:Reactome.
 GO:0060444; P:branching involved in mammary gland duct morphogenesis; IEA:Compara.
 GO:0071407; P:cellular response to organic cyclic compound; IEA:Compara.
 GO:0033598; P:mammary gland epithelial cell proliferation; IEA:Compara.
 GO:0043433; P:negative regulation of sequence-specific DNA binding transcription factor activity; TAS:BHF-UCL.
 GO:0045879; P:negative regulation of smoothened signaling pathway; TAS:BHF-UCL.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IMP:UniProtKB.
 GO:0042753; P:positive regulation of circadian rhythm; IEA:Compara.
 GO:0045862; P:positive regulation of proteolysis; IMP:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0051437; P:positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; TAS:Reactome.
 GO:0006470; P:protein dephosphorylation; IEA:Compara.
 GO:0031648; P:protein destabilization; IMP:UniProtKB.
 GO:0000209; P:protein polyubiquitination; IEA:Compara.
 GO:0016567; P:protein ubiquitination; IDA:UniProtKB.
 GO:0051726; P:regulation of cell cycle; IEA:Compara.
 GO:0043122; P:regulation of I-kappaB kinase/NF-kappaB cascade; IEA:Compara.
 GO:0061136; P:regulation of proteasomal protein catabolic process; IEA:Compara.
 GO:0007165; P:signal transduction; TAS:ProtInc.
 GO:0016032; P:viral reproduction; TAS:Reactome.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW.
 GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW. 
Interpro
 IPR021977; Beta-TrCP_D.
 IPR001810; F-box_dom_cyclin-like.
 IPR020472; G-protein_beta_WD-40_rep.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF12125; Beta-TrCP_D
 PF00400; WD40 
SMART
 SM01028; Beta-TrCP_D
 SM00256; FBOX
 SM00320; WD40 
PROSITE
 PS50181; FBOX
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.