UniProt Accession

 ACOT1_MOUSE; O55137; Q549A9 

Genbank Protein ID

 AF180795; AF180793; AF180794; Y14004; BC028261 

Genbank Nucleotide ID

 AAF13870.1; AAF13870.1; AAF13870.1; CAA74327.1; AAH28261.1 

Protein Name

 Acyl-coenzyme A thioesterase 1 

Protein Synonyms/Alias

 Acyl-CoA thioesterase 1; CTE-I; Inducible cytosolic acyl-coenzyme A thioester hydrolase; Long chain acyl-CoA thioester hydrolase; Long chain acyl-CoA hydrolase 

Gene Name

 Acot1 

Gene Synonyms/Alias

 Cte1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
42	RSVLRDEKGALFRAH	acetylation	[1, 2, 3, 4]
42	RSVLRDEKGALFRAH	succinylation	[3]
42	RSVLRDEKGALFRAH	ubiquitination	[5]
304	QSPLVDKKSFIPVER	acetylation	[6]
338	FYADEISKRLQAHGK	acetylation	[6]

Reference

 [1] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [6] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 

Functional Description

 Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. True acyl-CoA thioesterase being highly specific for saturated C12-C20 acyl-CoAs, with only a very low activity with decanoyl-CoA as substrate. Most active on myristoyl- and palmitoyl-CoA. Introduction of one or two double bonds decreases the activity to about half. No detectable activity with bile acid CoAs such as choloyl-CoA and chenodeoxycholoyl-CoA. 

Sequence Annotation

 ACT_SITE 232 232 Charge relay system.
 ACT_SITE 324 324 Charge relay system.
 ACT_SITE 358 358 Charge relay system.  

Keyword

 Complete proteome; Cytoplasm; Hydrolase; Reference proteome; Serine esterase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 419 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0047617; F:acyl-CoA hydrolase activity; IDA:MGI.
 GO:0004091; F:carboxylesterase activity; IEA:UniProtKB-KW.
 GO:0016290; F:palmitoyl-CoA hydrolase activity; IEA:EC.
 GO:0006637; P:acyl-CoA metabolic process; TAS:MGI.
 GO:0001676; P:long-chain fatty acid metabolic process; IDA:MGI. 

Interpro

 IPR016662; Acyl-CoA_thioEstase_long-chain.
 IPR014940; BAAT_C.
 IPR006862; Thio_Ohase/aa_AcTrfase. 

Pfam

 PF08840; BAAT_C
 PF04775; Bile_Hydr_Trans 

SMART

  

PROSITE

  

PRINTS