CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030498
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Equilibrative nucleoside transporter 1 
Protein Synonyms/Alias
 Solute carrier family 29 (Nucleoside transporters), member 1, isoform CRA_b; cDNA FLJ33172 fis, clone ADRGL2002029, highly similar to Equilibrative nucleoside transporter 1 
Gene Name
 SLC29A1 
Gene Synonyms/Alias
 hCG_19000 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
318YRYYQQLKLEGPGEQubiquitination[1, 2, 3, 4, 5, 6, 7, 8]
328GPGEQETKLDLISKGubiquitination[1, 2, 3, 4, 5, 6, 7, 8, 9, 10]
334TKLDLISKGEEPRAGubiquitination[1, 2, 3, 4, 5, 6, 7, 8, 10, 11]
342GEEPRAGKEESGVSVubiquitination[2, 3, 5, 6, 8, 10]
362TNESHSIKAILKNISubiquitination[2, 5]
435AVFMWPGKDSRWLPSubiquitination[1, 2, 6, 7]
460LLLLCNIKPRRYLTVubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [9] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [10] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [11] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 535 AA 
Protein Sequence
MRRERTRGPQ AWEFPSPTKS GCSLQSLSRD LRELREGEKP EDQAETEESW QGLARKTPGK 60
ACAPEGGSCQ PGKTENTITM TTSHQPQDRY KAVWLIFFML GLGTLLPWNF FMTATQYFTN 120
RLDMSQNVSL VTAELSKDAQ ASAAPAAPLP ERNSLSAIFN NVMTLCAMLP LLLFTYLNSF 180
LHQRIPQSVR ILGSLVAILL VFLITAILVK VQLDALPFFV ITMIKIVLIN SFGAILQGSL 240
FGLAGLLPAS YTAPIMSGQG LAGFFASVAM ICAIASGSEL SESAFGYFIT ACAVIILTII 300
CYLGLPRLEF YRYYQQLKLE GPGEQETKLD LISKGEEPRA GKEESGVSVS NSQPTNESHS 360
IKAILKNISV LAFSVCFIFT ITIGMFPAVT VEVKSSIAGS STWERYFIPV SCFLTFNIFD 420
WLGRSLTAVF MWPGKDSRWL PSLVLARLVF VPLLLLCNIK PRRYLTVVFE HDAWFIFFMA 480
AFAFSNGYLA SLCMCFGPKK VKPAEAETAG AIMAFFLCLG LALGAVFSFL FRAIV 535 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:InterPro.
 GO:0005337; F:nucleoside transmembrane transporter activity; IEA:InterPro. 
Interpro
 IPR002259; Eqnu_transpt. 
Pfam
 PF01733; Nucleoside_tran 
SMART
  
PROSITE
  
PRINTS
 PR01130; DERENTRNSPRT.