UniProt Accession

 UB2E1_MOUSE; P52482 

Genbank Protein ID

 X92665; BC003781 

Genbank Nucleotide ID

 CAA63353.1; AAH03781.1 

Protein Name

 Ubiquitin-conjugating enzyme E2 E1 

Protein Synonyms/Alias

 UbcM3; Ubiquitin carrier protein E1; Ubiquitin-protein ligase E1 

Gene Name

 Ube2e1 

Gene Synonyms/Alias

 Ubce5; Ubcm3 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
43	VSMSKNSKLLSTSAK	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes 'Lys-48'-linked polyubiquitination (By similarity). 

Sequence Annotation

 ACT_SITE 131 131 Glycyl thioester intermediate (By
 MOD_RES 2 2 N-acetylserine (By similarity).
 CROSSLNK 136 136 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; ATP-binding; Complete proteome; Isopeptide bond; Ligase; Nucleotide-binding; Nucleus; Reference proteome; Ubl conjugation; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 193 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0000151; C:ubiquitin ligase complex; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0042296; F:ISG15 ligase activity; IEA:Compara.
 GO:0004842; F:ubiquitin-protein ligase activity; ISS:UniProtKB.
 GO:0033523; P:histone H2B ubiquitination; IEA:Compara.
 GO:0010390; P:histone monoubiquitination; IEA:Compara.
 GO:0032020; P:ISG15-protein conjugation; IEA:Compara.
 GO:0070936; P:protein K48-linked ubiquitination; ISS:UniProtKB. 

Interpro

 IPR000608; UBQ-conjugat_E2.
 IPR023313; UBQ-conjugating_AS.
 IPR016135; UBQ-conjugating_enzyme/RWD. 

Pfam

 PF00179; UQ_con 

SMART

  

PROSITE

 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 

PRINTS