CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012869
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 AP-5 complex subunit beta-1 
Protein Synonyms/Alias
 Adapter-related protein complex 5 beta subunit; Beta5 
Gene Name
 AP5B1 
Gene Synonyms/Alias
 PP1030 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
44LSDLRSEKLSEQTKVubiquitination[1]
161LRELESCKPGLLGGSubiquitination[1, 2, 3, 4]
214LGGLLTDKVSPTGGGubiquitination[1, 2, 3, 5, 6]
309GQPPALFKPQLVRLLubiquitination[2, 4, 5, 7, 8]
431AEEEEEEKGQLPSPRubiquitination[1, 5]
674SLGSHRVKGPLPVLKubiquitination[1, 2, 4, 5, 7, 8]
681KGPLPVLKLQPEALEubiquitination[1, 2, 4, 5, 6, 7, 8]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 As part of AP-5, a probable fifth adapter protein complex it may be involved in endosomal transport. 
Sequence Annotation
  
Keyword
 Complete proteome; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 878 AA 
Protein Sequence
MGPLSRDAWA QRLGAFRASP SAFMAGPEGE DLGRDLLSDL RSEKLSEQTK VSLLALSMEY 60
PAQLWPDASA AEVAATSLLD TLVLLPPRPS ALRRPLLLAA TTALAAGGAL GPTSGASCRL 120
LPLLLGLAAG SDLGRGFVPA SEQRPLQATA CECLRELESC KPGLLGGSLG LLRGLLGQEG 180
PVQPLSLLLA LALRNTLVLQ SRVGAGLGGL LTDKVSPTGG GPWDWTLVEE GDGRLQPQAP 240
SWPAAEEGEG ERSLTAREHS PEEARELRAA VIQLLDTSYL LTPVAQAQLL WLLGWALRGL 300
QGQPPALFKP QLVRLLGTAQ LTLLHAMLAL KAAFGEALFT AQDEALLLRR LTLAAQHPAL 360
PPPTHLFYLH CVLSFPENWP LGPEGEEAAP LLLGPQLCRG LLPSLLHDPM ALLARLHLLC 420
LLCAEEEEEE KGQLPSPRHY LEELLAGLRQ RAALDGGPRA LATLCFQASY LVACCLAGQP 480
TVLTPLIHGL AQLYQARPML APHFVDLLDQ VDSELREPLK VVLRQVVVSR PGRDEALCWH 540
LQMLAKVADG DAQSATLNFL QAAAAHCTNW DLQQGLLRVC RALLRAGVRG GLVDLLQVLA 600
RQLEDPDGRD HARLYYILLA HLAAPKLGVA LGPSLAAPAL ASSLVAENQG FVAALMVQEA 660
PALVRLSLGS HRVKGPLPVL KLQPEALEPI YSLELRFRVE GQLYAPLEAV HVPCLCPGRP 720
ARPLLLPLQP RCPAPARLDV HALYTTSTGL TCHAHLPPLF VNFADLFLPF PQPPEGAGLG 780
FFEELWDSCL PEGAESRVWC PLGPQGLEGL VSRHLEPFVV VAQPPTSYCV AIHLPPDSKL 840
LLRLEAALAD GVPVALRTDD WAVLPLAGDY LRGLAAAV 878 
Gene Ontology
 GO:0030119; C:AP-type membrane coat adaptor complex; IDA:UniProtKB.
 GO:0016197; P:endosomal transport; IMP:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 
Interpro
  
Pfam
  
SMART
  
PROSITE
  
PRINTS