CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019679
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription factor 12 
Protein Synonyms/Alias
 TCF-12; Class B basic helix-loop-helix protein 20; bHLHb20; DNA-binding protein HTF4; E-box-binding protein; Transcription factor HTF-4 
Gene Name
 TCF12 
Gene Synonyms/Alias
 BHLHB20; HEB; HTF4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
110MNSNLMGKTSERGSFubiquitination[1]
151AQLSSSGKPGTAYYSubiquitination[1]
416QDAMSFLKDVCEQSRubiquitination[1]
595EDLNPEQKIEREKERubiquitination[1]
622RDINEAFKELGRMCQubiquitination[1]
666RERNLNPKAACLKRRubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). 
Sequence Annotation
 DOMAIN 577 630 bHLH.
 REGION 119 140 Leucine-zipper.
 REGION 632 655 Class A specific domain.
 MOTIF 19 27 9aaTAD.
 MOD_RES 67 67 Phosphoserine.
 MOD_RES 313 313 Phosphothreonine.
 MOD_RES 333 333 Phosphoserine.
 MOD_RES 557 557 Phosphothreonine.
 MOD_RES 559 559 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Developmental protein; Differentiation; DNA-binding; Neurogenesis; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 682 AA 
Protein Sequence
MNPQQQRMAA IGTDKELSDL LDFSAMFSPP VNSGKTRPTT LGSSQFSGSG IDERGGTTSW 60
GTSGQPSPSY DSSRGFTDSP HYSDHLNDSR LGAHEGLSPT PFMNSNLMGK TSERGSFSLY 120
SRDTGLPGCQ SSLLRQDLGL GSPAQLSSSG KPGTAYYSFS ATSSRRRPLH DSAALDPLQA 180
KKVRKVPPGL PSSVYAPSPN SDDFNRESPS YPSPKPPTSM FASTFFMQDG THNSSDLWSS 240
SNGMSQPGFG GILGTSTSHM SQSSSYGNLH SHDRLSYPPH SVSPTDINTS LPPMSSFHRG 300
STSSSPYVAA SHTPPINGSD SILGTRGNAA GSSQTGDALG KALASIYSPD HTSSSFPSNP 360
STPVGSPSPL TGTSQWPRPG GQAPSSPSYE NSLHSLKNRV EQQLHEHLQD AMSFLKDVCE 420
QSRMEDRLDR LDDAIHVLRN HAVGPSTSLP AGHSDIHSLL GPSHNAPIGS LNSNYGGSSL 480
VASSRSASMV GTHREDSVSL NGNHSVLSST VTTSSTDLNH KTQENYRGGL QSQSGTVVTT 540
EIKTENKEKD ENLHEPPSSD DMKSDDESSQ KDIKVSSRGR TSSTNEDEDL NPEQKIEREK 600
ERRMANNARE RLRVRDINEA FKELGRMCQL HLKSEKPQTK LLILHQAVAV ILSLEQQVRE 660
RNLNPKAACL KRREEEKVSA VSAEPPTTLP GTHPGLSETT NPMGHM 706 
Gene Ontology
 GO:0005634; C:nucleus; IDA:LIFEdb.
 GO:0005667; C:transcription factor complex; IEA:Compara.
 GO:0070888; F:E-box binding; IEA:Compara.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:BHF-UCL.
 GO:0006955; P:immune response; TAS:ProtInc.
 GO:0007517; P:muscle organ development; TAS:ProtInc.
 GO:0045666; P:positive regulation of neuron differentiation; IEA:Compara.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; TAS:ProtInc.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR011598; bHLH_dom. 
Pfam
 PF00010; HLH 
SMART
 SM00353; HLH 
PROSITE
 PS50888; BHLH 
PRINTS