UniProt Accession

 SEN34_HUMAN; Q9BSV6; A6NNB1; B0V3J1; Q9BVT1; Q9H6H5 

Genbank Protein ID

 AK025929; CU457734; CU151838; CH471135; BC004530; BC020805 

Genbank Nucleotide ID

 BAB15284.1; CAP19124.1; CAQ09596.1; EAW72203.1; AAH04530.1; AAH20805.1 

Protein Name

 tRNA-splicing endonuclease subunit Sen34 

Protein Synonyms/Alias

 Leukocyte receptor cluster member 5; tRNA-intron endonuclease Sen34; HsSen34 

Gene Name

 TSEN34 

Gene Synonyms/Alias

 LENG5; SEN34 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
87	SLALTSFKRQQEESF	ubiquitination	[1, 2]
115	RRQELLEKITEGQAA	ubiquitination	[1, 2]
207	LDWRVQSKDWPHAGR	ubiquitination	[1, 2]
286	RLGTSVRKTLLLCSP	ubiquitination	[2]

Reference

 [1] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'- end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. 

Sequence Annotation

 ACT_SITE 247 247 By similarity.
 ACT_SITE 255 255 By similarity.
 ACT_SITE 286 286 By similarity.  

Keyword

 Complete proteome; Disease mutation; Endonuclease; Hydrolase; mRNA processing; Nuclease; Nucleus; Polymorphism; Pontocerebellar hypoplasia; Reference proteome; tRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 310 AA 

Protein Sequence

Gene Ontology

 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0000214; C:tRNA-intron endonuclease complex; IEA:InterPro.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0000213; F:tRNA-intron endonuclease activity; IEA:EC.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC.
 GO:0000379; P:tRNA-type intron splice site recognition and cleavage; IEA:InterPro. 

Interpro

 IPR011856; tRNA_endonuc-like_dom.
 IPR006677; tRNA_intron_Endonuc_cat-like.
 IPR006676; tRNA_splic.
 IPR016690; tRNA_splic_SEN34. 

Pfam

 PF01974; tRNA_int_endo 

SMART

  

PROSITE

  

PRINTS