CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022461
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NCK-interacting protein with SH3 domain 
Protein Synonyms/Alias
 54 kDa VacA-interacting protein; 54 kDa vimentin-interacting protein; VIP54; 90 kDa SH3 protein interacting with Nck; AF3p21; Dia-interacting protein 1; DIP-1; Diaphanous protein-interacting protein; SH3 adapter protein SPIN90; WASP-interacting SH3-domain protein; WISH; Wiskott-Aldrich syndrome protein-interacting protein 
Gene Name
 NCKIPSD 
Gene Synonyms/Alias
 AF3P21; SPIN90 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
84TAMRDGGKYSLEQRGubiquitination[1]
95EQRGVLQKLIHHRKEubiquitination[2]
589NVKIFSEKLLLLLNRubiquitination[3]
712MIVREMCKEFLVLGEubiquitination[2]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1). Induces microspike formation, in vivo (By similarity). In vitro, stimulates N-WASP- induced ARP2/3 complex activation in the absence of CDC42 (By similarity). May play an important role in the maintenance of sarcomeres and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion. Plays a role in angiogenesis. 
Sequence Annotation
 DOMAIN 1 58 SH3.
 MOTIF 175 192 Nuclear localization signal (Potential).  
Keyword
 Alternative splicing; Chromosomal rearrangement; Complete proteome; Nucleus; Polymorphism; Proto-oncogene; Reference proteome; SH3 domain; SH3-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 722 AA 
Protein Sequence
MYRALYAFRS AEPNALAFAA GETFLVLERS SAHWWLAARA RSGETGYVPP AYLRRLQGLE 60
QDVLQAIDRA IEAVHNTAMR DGGKYSLEQR GVLQKLIHHR KETLSRRGPS ASSVAVMTSS 120
TSDHHLDAAA ARQPNGVCRA GFERQHSLPS SEHLGADGGL YQIPLPSSQI PPQPRRAAPT 180
TPPPPVKRRD REALMASGSG GHNTMPSGGN SVSSGSSVSS TSLDTLYTSS SPSEPGSSCS 240
PTPPPVPRRG THTTVSQVQP PPSKASAPEP PAEEEVATGT TSASDDLEAL GTLSLGTTEE 300
KAAAEAAVPR TIGAELMELV RRNTGLSHEL CRVAIGIIVG HIQASVPASS PVMEQVLLSL 360
VEGKDLSMAL PSGQVCHDQQ RLEVIFADLA RRKDDAQQRS WALYEDEGVI RCYLEELLHI 420
LTDADPEVCK KMCKRNEFES VLALVAYYQM EHRASLRLLL LKCFGAMCSL DAAIISTLVS 480
SVLPVELARD MQTDTQDHQK LCYSALILAM VFSMGEAVPY AHYEHLGTPF AQFLLNIVED 540
GLPLDTTEQL PDLCVNLLLA LNLHLPAADQ NVIMAALSKH ANVKIFSEKL LLLLNRGDDP 600
VRIFKHEPQP PHSVLKFLQD VFGSPATAAI FYHTDMMALI DITVRHIADL SPGDKLRMEY 660
LSLMHAIVRT TPYLQHRHRL PDLQAILRRI LNEEETSPQC QMDRMIVREM CKEFLVLGEA 720
PS 722 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005882; C:intermediate filament; NAS:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0008092; F:cytoskeletal protein binding; NAS:UniProtKB.
 GO:0007010; P:cytoskeleton organization; NAS:UniProtKB.
 GO:0038096; P:Fc-gamma receptor signaling pathway involved in phagocytosis; TAS:Reactome.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0006607; P:NLS-bearing substrate import into nucleus; TAS:ProtInc. 
Interpro
 IPR018556; DUF2013.
 IPR001452; SH3_domain. 
Pfam
 PF09431; DUF2013
 PF00018; SH3_1 
SMART
 SM00326; SH3 
PROSITE
 PS50002; SH3 
PRINTS