CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008098
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA polymerase delta subunit 2 
Protein Synonyms/Alias
 DNA polymerase delta subunit p50 
Gene Name
 POLD2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
80WGSGVGVKKLCELQPubiquitination[1, 2]
81GSGVGVKKLCELQPEubiquitination[2]
90CELQPEEKCCVVGTLubiquitination[2]
99CVVGTLFKAMPLQPSubiquitination[2, 3]
125LPQPPRSKYIHPDDEubiquitination[1, 2, 3, 4]
145ELQRIKLKGTIDVSKubiquitination[1, 2, 4]
267KAKYLTKKTQAASVEubiquitination[1, 2]
389LGCYPFYKTDPFIFPubiquitination[2, 5, 6]
413NTPSFGSKIIRGPEDubiquitination[2, 6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 The function of the small subunit is not yet clear. 
Sequence Annotation
 MOD_RES 15 15 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; DNA replication; DNA-directed DNA polymerase; Nucleotidyltransferase; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 469 AA 
Protein Sequence
MFSEQAAQRA HTLLSPPSAN NATFARVPVA TYTNSSQPFR LGERSFSRQY AHIYATRLIQ 60
MRPFLENRAQ QHWGSGVGVK KLCELQPEEK CCVVGTLFKA MPLQPSILRE VSEEHNLLPQ 120
PPRSKYIHPD DELVLEDELQ RIKLKGTIDV SKLVTGTVLA VFGSVRDDGK FLVEDYCFAD 180
LAPQKPAPPL DTDRFVLLVS GLGLGGGGGE SLLGTQLLVD VVTGQLGDEG EQCSAAHVSR 240
VILAGNLLSH STQSRDSINK AKYLTKKTQA ASVEAVKMLD EILLQLSASV PVDVMPGEFD 300
PTNYTLPQQP LHPCMFPLAT AYSTLQLVTN PYQATIDGVR FLGTSGQNVS DIFRYSSMED 360
HLEILEWTLR VRHISPTAPD TLGCYPFYKT DPFIFPECPH VYFCGNTPSF GSKIIRGPED 420
QTVLLVTVPD FSATQTACLV NLRSLACQPI SFSGFGAEDD DLGGLGLGP 469 
Gene Ontology
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
 GO:0006284; P:base-excision repair; TAS:Reactome.
 GO:0006271; P:DNA strand elongation involved in DNA replication; TAS:Reactome.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome.
 GO:0006297; P:nucleotide-excision repair, DNA gap filling; TAS:Reactome.
 GO:0000722; P:telomere maintenance via recombination; TAS:Reactome.
 GO:0032201; P:telomere maintenance via semi-conservative replication; TAS:Reactome.
 GO:0006283; P:transcription-coupled nucleotide-excision repair; TAS:Reactome. 
Interpro
 IPR007185; DNA_pol_alpha/epsilon_bsu.
 IPR024826; DNA_pol_delta/II_ssu. 
Pfam
 PF04042; DNA_pol_E_B 
SMART
  
PROSITE
  
PRINTS