UniProt Accession

 E9PUY9_MOUSE; E9PUY9 

Genbank Protein ID

 AC124537 

Genbank Nucleotide ID

  

Protein Name

 Enoyl-CoA delta isomerase 2, mitochondrial 

Protein Synonyms/Alias

  

Gene Name

 Eci2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
16	ENALNQVKLLKKDPG	acetylation	[1, 2, 3, 4, 5, 6]
16	ENALNQVKLLKKDPG	succinylation	[5]
16	ENALNQVKLLKKDPG	ubiquitination	[7]
19	LNQVKLLKKDPGNEV	acetylation	[4]
20	NQVKLLKKDPGNEVK	acetylation	[4, 5]
20	NQVKLLKKDPGNEVK	succinylation	[5]
27	KDPGNEVKLRLYALY	acetylation	[4, 5, 6]
27	KDPGNEVKLRLYALY	succinylation	[5]
35	LRLYALYKQATEGPC	acetylation	[4, 5]
35	LRLYALYKQATEGPC	succinylation	[5]
46	EGPCNMPKPGMLDFV	acetylation	[4, 5, 6, 8]
46	EGPCNMPKPGMLDFV	succinylation	[5]
55	GMLDFVNKAKWDAWN	acetylation	[4, 5, 6]
55	GMLDFVNKAKWDAWN	succinylation	[5]
57	LDFVNKAKWDAWNAL	acetylation	[2, 3, 4, 5, 6, 8, 9]
57	LDFVNKAKWDAWNAL	succinylation	[5]
57	LDFVNKAKWDAWNAL	ubiquitination	[7]
94	SEAPSQGKRGADEKA	acetylation	[4, 5, 6]
94	SEAPSQGKRGADEKA	succinylation	[5]
100	GKRGADEKARESKDI	acetylation	[6]
105	DEKARESKDILVTSE	acetylation	[4, 5, 6, 8, 10]
105	DEKARESKDILVTSE	succinylation	[5]
125	ITFNRPTKKNAISFQ	acetylation	[4, 6]
126	TFNRPTKKNAISFQM	acetylation	[4, 5]
126	TFNRPTKKNAISFQM	succinylation	[5]
126	TFNRPTKKNAISFQM	ubiquitination	[7]

Reference

 [1] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [6] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [7] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [8] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [9] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [10] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599] 

Functional Description

  

Sequence Annotation

  

Keyword

 Complete proteome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 202 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005777; C:peroxisome; IEA:Compara.
 GO:0000062; F:fatty-acyl-CoA binding; IEA:InterPro.
 GO:0016863; F:intramolecular oxidoreductase activity, transposing C=C bonds; IEA:Compara.
 GO:0006635; P:fatty acid beta-oxidation; IEA:Compara. 

Interpro

 IPR022408; Acyl-CoA-binding_prot_CS.
 IPR000582; Acyl-CoA-binding_protein.
 IPR001753; Crotonase_core_superfam.
 IPR014352; FERM/acyl-CoA-bd_prot_3-hlx. 

Pfam

 PF00887; ACBP
 PF00378; ECH 

SMART

  

PROSITE

 PS00880; ACB_1
 PS51228; ACB_2 

PRINTS

 PR00689; ACOABINDINGP.