CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031101
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 2 subunit 1 
Protein Synonyms/Alias
 cDNA FLJ58035, highly similar to Homo sapiens eukaryotic translation initiation factor (eIF) 2A (eIF2A), mRNA 
Gene Name
 EIF2A 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
75WQPYTTSKDGTAGIPubiquitination[1]
90NLQLYDVKTGTCLKSubiquitination[2, 3]
96VKTGTCLKSFIQKKMubiquitination[3, 4]
102LKSFIQKKMQNWCPSubiquitination[3]
139NFNTIANKLHLQKINubiquitination[1, 3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Initiation factor; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 560 AA 
Protein Sequence
MAPSTPLLTV RGSEGLYMVN GPPHFTESTV FPRVNIISVT NKGLLHSFDL LKAVCLEFSP 60
KNTVLATWQP YTTSKDGTAG IPNLQLYDVK TGTCLKSFIQ KKMQNWCPSW SEDETLCARN 120
VNNEVHFFEN NNFNTIANKL HLQKINDFVL SPGPQPYKVA VYVPGSKGAP SFVRLYQYPN 180
FAGPHAALAN KSFFKADKVT MLWNKKATAV LVIASTDVDK TGASYYGEQT LHYIATNGES 240
AVVQLPKNGP IYDVVWNSSS TEFCAVYGFM PAKATIFNLK CDPVFDFGTG PRNAAYYSPH 300
GHILVLAGFG NLRGQMEVWD VKNYKLISKP VASDSTYFAW CPDGEHILTA TCAPRLRVNN 360
GYKIWHYTGS ILHKYDVPSN AELWQVSWQP FLDGIFPAKT ITYQAVPSEV PNEEPKVATA 420
YRPPALRNKP ITNSKLHEEE PPQNMKPQSG NDKPLSKTAL KNQRKHEAKK AAKQEARSDK 480
SPDLAPTPAP QSTPRNTVSQ SISGDPEIDK KIKNLKKKLK AIEQLKEQAA TGKQLEKNQL 540
EKIQKETALL QELEDLELGI 560 
Gene Ontology
 GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW. 
Interpro
 IPR011387; TIF2A.
 IPR013979; TIF_beta_prop-like.
 IPR015943; WD40/YVTN_repeat-like_dom. 
Pfam
 PF08662; eIF2A 
SMART
  
PROSITE
  
PRINTS