CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004356
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phenylalanine--tRNA ligase alpha subunit 
Protein Synonyms/Alias
 Phenylalanyl-tRNA synthetase alpha subunit; PheRS 
Gene Name
 FRS2 
Gene Synonyms/Alias
 YFL022C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
93QIKDVMSKLGPQVGKacetylation[1]
206MVSTNAYKDLKFKPYubiquitination[2]
211AYKDLKFKPYNFNSQacetylation[1]
232GALHPLNKVREEFRQubiquitination[2]
301KTYMDNIKAVHEQGRacetylation[1]
319IGYRYNWKPEECQKLacetylation[1]
470LERPTMIKYKVQNIRubiquitination[2]
483IRELLGHKVSLDFIEubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
  
Sequence Annotation
 REGION 2 173 Contains the major tRNA-Phe binding
 MOD_RES 2 2 N-acetylserine.  
Keyword
 Acetylation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 503 AA 
Protein Sequence
MSDFQLEILK KLDELDEIKS TLATFPQHGS QDVLSALNSL KAHNKLEFSK VDTVTYDLTK 60
EGAQILNEGS YEIKLVKLIQ ELGQLQIKDV MSKLGPQVGK VGQARAFKNG WIAKNASNEL 120
ELSAKLQNTD LNELTDETQS ILAQIKNNSH LDSIDAKILN DLKKRKLIAQ GKITDFNVTK 180
GPEFSTDLTK LETDLTSDMV STNAYKDLKF KPYNFNSQGV QISSGALHPL NKVREEFRQI 240
FFSMGFTEMP SNQYVETGFW NFDALYVPQQ HPARDLQDTF YIKDPLTADL PDDKTYMDNI 300
KAVHEQGRFG SIGYRYNWKP EECQKLVLRT HSTAISARML HDLAKDPKPT RLFSIDRVFR 360
NEAVDATHLA EFHQVEGVLA DYNITLGDLI KFMEEFFERM GVTGLRFKPT YNPYTEPSME 420
IFSWHEGLQK WVEIGNSGMF RPEMLESMGL PKDLRVLGWG LSLERPTMIK YKVQNIRELL 480
GHKVSLDFIE TNPAARLDED LYE 503 
Gene Ontology
 GO:0009328; C:phenylalanine-tRNA ligase complex; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004826; F:phenylalanine-tRNA ligase activity; IEA:EC.
 GO:0000049; F:tRNA binding; IEA:InterPro.
 GO:0006432; P:phenylalanyl-tRNA aminoacylation; IDA:SGD. 
Interpro
 IPR006195; aa-tRNA-synth_II.
 IPR004529; Phe-tRNA-synth_IIc_asu.
 IPR002319; Phenylalanyl-tRNA_Synthase. 
Pfam
 PF01409; tRNA-synt_2d 
SMART
  
PROSITE
 PS50862; AA_TRNA_LIGASE_II 
PRINTS