CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006528
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Peroxiredoxin-1 
Protein Synonyms/Alias
 Macrophage 23 kDa stress protein; Osteoblast-specific factor 3; OSF-3; Thioredoxin peroxidase 2; Thioredoxin-dependent peroxide reductase 2 
Gene Name
 Prdx1 
Gene Synonyms/Alias
 Msp23; Paga; Tdpx2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
7*MSSGNAKIGYPAPNubiquitination[1]
16GYPAPNFKATAVMPDubiquitination[1]
27VMPDGQFKDISLSEYacetylation[2]
27VMPDGQFKDISLSEYubiquitination[1]
35DISLSEYKGKYVVFFacetylation[2]
35DISLSEYKGKYVVFFubiquitination[1]
93AWINTPKKQGGLGPMubiquitination[1]
109IPLISDPKRTIAQDYacetylation[2]
109IPLISDPKRTIAQDYubiquitination[1]
120AQDYGVLKADEGISFubiquitination[1]
136GLFIIDDKGILRQITacetylation[2]
136GLFIIDDKGILRQITubiquitination[1]
168QAFQFTDKHGEVCPAacetylation[3, 4]
168QAFQFTDKHGEVCPAubiquitination[1]
178EVCPAGWKPGSDTIKacetylation[2, 3, 5, 6]
185KPGSDTIKPDVNKSKacetylation[2, 3, 4, 7]
185KPGSDTIKPDVNKSKubiquitination[1]
190TIKPDVNKSKEYFSKacetylation[4]
190TIKPDVNKSKEYFSKubiquitination[1]
192KPDVNKSKEYFSKQKacetylation[3]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [5] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [6] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [7] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599] 
Functional Description
 Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Regulates GDPD5 function by reducing an intramolecular disulfide bond (By similarity). Cooperates with GDPD5 to drive postmitotic motor neuron differentiation. 
Sequence Annotation
 DOMAIN 6 165 Thioredoxin.
 ACT_SITE 52 52 Cysteine sulfenic acid (-SOH)
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 7 7 N6-acetyllysine (By similarity).
 MOD_RES 10 10 Phosphotyrosine.
 MOD_RES 16 16 N6-acetyllysine (By similarity).
 MOD_RES 27 27 N6-acetyllysine (By similarity).
 MOD_RES 35 35 N6-acetyllysine (By similarity).
 MOD_RES 90 90 Phosphothreonine (By similarity).
 DISULFID 52 52 Interchain (with C-173); in linked form
 DISULFID 173 173 Interchain (with C-52); in linked form  
Keyword
 Acetylation; Antioxidant; Complete proteome; Cytoplasm; Direct protein sequencing; Disulfide bond; Oxidoreductase; Peroxidase; Phosphoprotein; Redox-active center; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 199 AA 
Protein Sequence
MSSGNAKIGY PAPNFKATAV MPDGQFKDIS LSEYKGKYVV FFFYPLDFTF VCPTEIIAFS 60
DRADEFKKLN CQVIGASVDS HFCHLAWINT PKKQGGLGPM NIPLISDPKR TIAQDYGVLK 120
ADEGISFRGL FIIDDKGILR QITINDLPVG RSVDEIIRLV QAFQFTDKHG EVCPAGWKPG 180
SDTIKPDVNK SKEYFSKQK 199 
Gene Ontology
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0005759; C:mitochondrial matrix; IEA:Compara.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005719; C:nuclear euchromatin; IEA:Compara.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0005782; C:peroxisomal matrix; IEA:Compara.
 GO:0020037; F:heme binding; IEA:Compara.
 GO:0008379; F:thioredoxin peroxidase activity; IEA:Compara.
 GO:0008283; P:cell proliferation; IMP:MGI.
 GO:0034101; P:erythrocyte homeostasis; IMP:MGI.
 GO:0042744; P:hydrogen peroxide catabolic process; IEA:Compara.
 GO:0042267; P:natural killer cell mediated cytotoxicity; IMP:MGI.
 GO:0042345; P:regulation of NF-kappaB import into nucleus; IMP:MGI.
 GO:0032872; P:regulation of stress-activated MAPK cascade; IMP:MGI.
 GO:0019430; P:removal of superoxide radicals; IMP:MGI. 
Interpro
 IPR000866; AhpC/TSA.
 IPR024706; Peroxiredoxin_AhpC-typ.
 IPR019479; Peroxiredoxin_C.
 IPR012336; Thioredoxin-like_fold. 
Pfam
 PF10417; 1-cysPrx_C
 PF00578; AhpC-TSA 
SMART
  
PROSITE
 PS51352; THIOREDOXIN_2 
PRINTS