CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004497
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cysteine synthase B 
Protein Synonyms/Alias
 CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B 
Gene Name
 cysM 
Gene Synonyms/Alias
 b2421; JW2414 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
41NNPAGSVKDRAALSMacetylation[1]
88ALKGYRMKLLMPDNMacetylation[1]
115AELILVTKEQGMEGAacetylation[1]
191RFMREQSKPVTIVGLacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Two cysteine synthase enzymes are found. Both catalyze the same reaction. Cysteine synthase B can also use thiosulfate in place of sulfide to give cysteine thiosulfonate as a product. 
Sequence Annotation
 REGION 174 178 Pyridoxal phosphate binding.
 BINDING 71 71 Pyridoxal phosphate.
 BINDING 255 255 Pyridoxal phosphate.
 MOD_RES 41 41 N6-(pyridoxal phosphate)lysine.  
Keyword
 3D-structure; Amino-acid biosynthesis; Complete proteome; Cysteine biosynthesis; Pyridoxal phosphate; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 303 AA 
Protein Sequence
MSTLEQTIGN TPLVKLQRMG PDNGSEVWLK LEGNNPAGSV KDRAALSMIV EAEKRGEIKP 60
GDVLIEATSG NTGIALAMIA ALKGYRMKLL MPDNMSQERR AAMRAYGAEL ILVTKEQGME 120
GARDLALEMA NRGEGKLLDQ FNNPDNPYAH YTTTGPEIWQ QTGGRITHFV SSMGTTGTIT 180
GVSRFMREQS KPVTIVGLQP EEGSSIPGIR RWPTEYLPGI FNASLVDEVL DIHQRDAENT 240
MRELAVREGI FCGVSSGGAV AGALRVAKAN PDAVVVAIIC DRGDRYLSTG VFGEEHFSQG 300
AGI 303 
Gene Ontology
 GO:0004124; F:cysteine synthase activity; IDA:EcoCyc.
 GO:0080146; F:L-cysteine desulfhydrase activity; IMP:EcoCyc.
 GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
 GO:0006535; P:cysteine biosynthetic process from serine; IDA:EcoCyc. 
Interpro
 IPR001216; Cys_synth_BS.
 IPR005856; Cys_synthKM.
 IPR005858; CysM.
 IPR001926; Trp_syn_b_sub_like_PLP_eny_SF. 
Pfam
 PF00291; PALP 
SMART
  
PROSITE
 PS00901; CYS_SYNTHASE 
PRINTS