UniProt Accession

 ANM2_HUMAN; P55345; P78350; Q498Y5; Q6FHF0; Q99781; Q9BW15; Q9UMC2 

Genbank Protein ID

 X99209; U80213; U79286; CR541804; AK123650; AP000339; AP000340; CH471079; BC000727; BC100026; AL109794 

Genbank Nucleotide ID

 CAA67599.1; AAB48437.1; AAB50221.1; CAG46603.1; BAG53931.1; EAX09265.1; AAH00727.1; AAI00027.1; CAB52454.1 

Protein Name

 Protein arginine N-methyltransferase 2 

Protein Synonyms/Alias

 Histone-arginine N-methyltransferase PRMT2 

Gene Name

 PRMT2 

Gene Synonyms/Alias

 HMT1; HRMT1L1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
56	LSFLRGEKILILRQT	ubiquitination	[1]
86	IPANHVGKHVDEYDP	ubiquitination	[1]
124	ADQPRTTKYHSVILQ	ubiquitination	[1]
427	TSQKVGEKVFPIWR*	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1- dependent manner). May be involved in growth regulation. 

Sequence Annotation

 DOMAIN 30 89 SH3.
 REGION 83 207 Interaction with RB1 (By similarity).
 REGION 133 275 Interaction with ESR1.
 ACT_SITE 211 211 By similarity.
 ACT_SITE 220 220 By similarity.
 BINDING 112 112 S-adenosyl-L-methionine (By similarity).
 BINDING 121 121 S-adenosyl-L-methionine (By similarity).
 BINDING 145 145 S-adenosyl-L-methionine; via carbonyl
 BINDING 168 168 S-adenosyl-L-methionine (By similarity).
 BINDING 197 197 S-adenosyl-L-methionine (By similarity).  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Methyltransferase; Nucleus; Reference proteome; S-adenosyl-L-methionine; SH3 domain; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 433 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0030331; F:estrogen receptor binding; IDA:UniProtKB.
 GO:0008469; F:histone-arginine N-methyltransferase activity; ISS:UniProtKB.
 GO:0004871; F:signal transducer activity; TAS:ProtInc.
 GO:0003713; F:transcription coactivator activity; IDA:UniProtKB.
 GO:0048588; P:developmental cell growth; ISS:UniProtKB.
 GO:0097194; P:execution phase of apoptosis; IDA:UniProtKB.
 GO:2000134; P:negative regulation of G1/S transition of mitotic cell cycle; ISS:UniProtKB.
 GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; IDA:UniProtKB.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0060765; P:regulation of androgen receptor signaling pathway; IDA:UniProtKB. 

Interpro

 IPR025799; Arg_MeTrfase.
 IPR025798; Arg_MeTrfase_PMTR2.
 IPR001452; SH3_domain. 

Pfam

 PF05185; PRMT5
 PF00018; SH3_1 

SMART

 SM00326; SH3 

PROSITE

 PS50002; SH3 

PRINTS

 PR00452; SH3DOMAIN.