UniProt Accession

 PUR9_RAT; O35567; Q6IN16 

Genbank Protein ID

 D89514; BC072496 

Genbank Nucleotide ID

 BAA22837.1; AAH72496.1 

Protein Name

 Bifunctional purine biosynthesis protein PURH 

Protein Synonyms/Alias

 Phosphoribosylaminoimidazolecarboxamide formyltransferase; 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase 

Gene Name

 Atic 

Gene Synonyms/Alias

 Purh 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
266	IPAAASFKHVSPAGA	acetylation	[1]
352	GYEEEALKILSKKKN	acetylation	[1]
356	EALKILSKKKNGSYC	acetylation	[1]
477	HPRVLSMKFKAGVKR	acetylation	[1]
507	GEGEDLVKWKALFEE	acetylation	[1]
524	ELLTEAEKKEWVDKL	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Bifunctional enzyme that catalyzes 2 steps in purine biosynthesis (By similarity). 

Sequence Annotation

 NP_BIND 12 14 IMP (By similarity).
 NP_BIND 34 37 IMP (By similarity).
 NP_BIND 64 67 IMP (By similarity).
 NP_BIND 101 104 IMP (By similarity).
 NP_BIND 125 127 IMP (By similarity).
 REGION 207 208 AICAR binding (By similarity).
 ACT_SITE 137 137 Proton acceptor (Potential).
 ACT_SITE 267 267 Proton acceptor (Probable).
 BINDING 316 316 AICAR; via carbonyl oxygen (By
 BINDING 339 339 AICAR (By similarity).
 BINDING 431 431 AICAR; shared with dimeric partner (By
 BINDING 451 451 AICAR; shared with dimeric partner (By
 BINDING 541 541 AICAR; via carbonyl oxygen; shared with
 BINDING 588 588 AICAR; shared with dimeric partner (By
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 199 199 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; Hydrolase; Multifunctional enzyme; Purine biosynthesis; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 592 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0003937; F:IMP cyclohydrolase activity; IDA:RGD.
 GO:0004643; F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity; IDA:RGD.
 GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
 GO:0006189; P:'de novo' IMP biosynthetic process; IDA:RGD.
 GO:0003360; P:brainstem development; IEP:RGD.
 GO:0021549; P:cerebellum development; IEP:RGD.
 GO:0021987; P:cerebral cortex development; IEP:RGD.
 GO:0009235; P:cobalamin metabolic process; TAS:RGD.
 GO:0046452; P:dihydrofolate metabolic process; IDA:RGD.
 GO:0009116; P:nucleoside metabolic process; IDA:RGD.
 GO:0031100; P:organ regeneration; IEP:RGD.
 GO:0010035; P:response to inorganic substance; IEP:RGD.
 GO:0046654; P:tetrahydrofolate biosynthetic process; IDA:RGD. 

Interpro

 IPR024051; AICAR_Tfase_dom.
 IPR024050; AICAR_Tfase_insert_dom.
 IPR002695; AICARFT_IMPCHas.
 IPR016193; Cytidine_deaminase-like.
 IPR011607; MGS-like_dom. 

Pfam

 PF01808; AICARFT_IMPCHas
 PF02142; MGS 

SMART

 SM00798; AICARFT_IMPCHas
 SM00851; MGS 

PROSITE

  

PRINTS