UniProt Accession

 UBE2S_MOUSE; Q921J4; Q3U4V7; Q8CF67 

Genbank Protein ID

 AK003078; AK017648; AK146276; AK154026; AK154067; BC012255; BC030171; BC083323 

Genbank Nucleotide ID

 BAC25019.1; BAC25523.1; BAE27035.1; BAE32323.1; BAE32353.1; AAH12255.1; AAH30171.1; AAH83323.1 

Protein Name

 Ubiquitin-conjugating enzyme E2 S 

Protein Synonyms/Alias

 Ubiquitin carrier protein S; Ubiquitin-conjugating enzyme E2-24 kDa; Ubiquitin-conjugating enzyme E2-EPF5; Ubiquitin-protein ligase S 

Gene Name

 Ube2s 

Gene Synonyms/Alias

 E2epf 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
100	EICVNVLKRDWTAEL	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination. 

Sequence Annotation

 ACT_SITE 95 95 Glycyl thioester intermediate (By
 MOD_RES 1 1 N-acetylmethionine (By similarity).  

Keyword

 Acetylation; ATP-binding; Cell cycle; Cell division; Complete proteome; Ligase; Nucleotide-binding; Reference proteome; Ubl conjugation; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 223 AA 

Protein Sequence

Gene Ontology

 GO:0005680; C:anaphase-promoting complex; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004842; F:ubiquitin-protein ligase activity; ISS:UniProtKB.
 GO:0051488; P:activation of anaphase-promoting complex activity; ISS:UniProtKB.
 GO:0031145; P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0010458; P:exit from mitosis; ISS:UniProtKB.
 GO:0010994; P:free ubiquitin chain polymerization; ISS:UniProtKB.
 GO:0070979; P:protein K11-linked ubiquitination; ISS:UniProtKB.
 GO:0044314; P:protein K27-linked ubiquitination; ISS:UniProtKB.
 GO:0035519; P:protein K29-linked ubiquitination; ISS:UniProtKB.
 GO:0085020; P:protein K6-linked ubiquitination; ISS:UniProtKB.
 GO:0070534; P:protein K63-linked ubiquitination; ISS:UniProtKB. 

Interpro

 IPR000608; UBQ-conjugat_E2.
 IPR023313; UBQ-conjugating_AS.
 IPR016135; UBQ-conjugating_enzyme/RWD. 

Pfam

 PF00179; UQ_con 

SMART

  

PROSITE

 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 

PRINTS