UniProt Accession

 UCHL1_RAT; Q00981; Q6P9V8 

Genbank Protein ID

 D10699; BC060573 

Genbank Nucleotide ID

 BAA01541.1; AAH60573.1 

Protein Name

 Ubiquitin carboxyl-terminal hydrolase isozyme L1 

Protein Synonyms/Alias

 UCH-L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1 

Gene Name

 Uchl1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
4	****MQLKPMEINPE	acetylation	[1]
4	****MQLKPMEINPE	ubiquitination	[2]
15	INPEMLNKVLAKLGV	acetylation	[1]
19	MLNKVLAKLGVAGQW	acetylation	[1]
71	KKQIEELKGQEVSPK	acetylation	[1]
71	KKQIEELKGQEVSPK	ubiquitination	[2]
78	KGQEVSPKVYFMKQT	acetylation	[1]
123	QFLSETEKLSPEDRA	acetylation	[1]
123	QFLSETEKLSPEDRA	ubiquitination	[2]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity (By similarity). 

Sequence Annotation

 REGION 5 10 Interaction with ubiquitin (By
 REGION 151 156 Interaction with ubiquitin (By
 REGION 211 216 Interaction with ubiquitin (By
 ACT_SITE 90 90 Nucleophile (By similarity).
 ACT_SITE 161 161 Proton donor (By similarity).
 LIPID 220 220 S-farnesyl cysteine (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Direct protein sequencing; Endoplasmic reticulum; Glycoprotein; Hydrolase; Ligase; Lipoprotein; Membrane; Oxidation; Prenylation; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 223 AA 

Protein Sequence

Gene Ontology

 GO:0030424; C:axon; IEA:Compara.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0004197; F:cysteine-type endopeptidase activity; IEA:Compara.
 GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
 GO:0008242; F:omega peptidase activity; IEA:Compara.
 GO:0004221; F:ubiquitin thiolesterase activity; IEA:Compara.
 GO:0007628; P:adult walking behavior; IEA:Compara.
 GO:0007412; P:axon target recognition; IEA:Compara.
 GO:0019896; P:axon transport of mitochondrion; IEA:Compara.
 GO:0008283; P:cell proliferation; IEA:Compara.
 GO:0042755; P:eating behavior; IEA:Compara.
 GO:0048747; P:muscle fiber development; IEA:Compara.
 GO:0043407; P:negative regulation of MAP kinase activity; IEA:Compara.
 GO:0050905; P:neuromuscular process; IEA:Compara.
 GO:0016579; P:protein deubiquitination; IEA:Compara.
 GO:0006950; P:response to stress; IEA:Compara.
 GO:0019233; P:sensory perception of pain; IDA:RGD.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IDA:RGD. 

Interpro

 IPR001578; Peptidase_C12. 

Pfam

 PF01088; Peptidase_C12 

SMART

  

PROSITE

 PS00140; UCH_1 

PRINTS

 PR00707; UBCTHYDRLASE.