| Tag | Content |
|---|
| CPLM ID | CPLM-011524 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | mRNA-binding protein PUF3 |
Protein Synonyms/Alias | Pumilio homology domain family member 3 |
Gene Name | PUF3 |
Gene Synonyms/Alias | YLL013C; L1325 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 528 | LRNSSSDKNSNSNMS | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | RNA-binding protein involved in post-transcriptional regulation. Negatively regulates expression of COX17 by binding to the 3'-UTR of COX17 mRNA. Promotes decay of COX17 mRNA by enhancing its rate of deadenylation and subsequent turnover. Predominantly binds to mRNAs encoding mitochondrial proteins and localizes them to the vicinity of mitochondria for translation. Regulates mitochondrial biogenesis, motility and morphology. |
Sequence Annotation | DOMAIN 513 871 PUM-HD.
REPEAT 538 573 Pumilio 1.
REPEAT 574 609 Pumilio 2.
REPEAT 610 645 Pumilio 3.
REPEAT 646 681 Pumilio 4.
REPEAT 682 717 Pumilio 5.
REPEAT 718 759 Pumilio 6.
REPEAT 760 795 Pumilio 7.
REPEAT 807 844 Pumilio 8.
MOD_RES 83 83 Phosphothreonine.
MOD_RES 210 210 Phosphoserine. |
Keyword | 3D-structure; Complete proteome; Cytoplasm; Membrane; Mitochondrion; Mitochondrion outer membrane; Phosphoprotein; Reference proteome; Repeat; RNA-binding. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 879 AA |
Protein Sequence | MEMNMDMDMD MELASIVSSL SALSHSNNNG GQAAAAGIVN GGAAGSQQIG GFRRSSFTTA 60
NEVDSEILLL HGSSESSPIF KKTALSVGTA PPFSTNSKKF FGNGGNYYQY RSTDTASLSS 120
ASYNNYHTHH TAANLGKNNK VNHLLGQYSA SIAGPVYYNG NDNNNSGGEG FFEKFGKSLI 180
DGTRELESQD RPDAVNTQSQ FISKSVSNAS LDTQNTFEQN VESDKNFNKL NRNTTNSGSL 240
YHSSSNSGSS ASLESENAHY PKRNIWNVAN TPVFRPSNNP AAVGATNVAL PNQQDGPANN 300
NFPPYMNGFP PNQFHQGPHY QNFPNYLIGS PSNFISQMIS VQIPANEDTE DSNGKKKKKA 360
NRPSSVSSPS SPPNNSPFPF AYPNPMMFMP PPPLSAPQQQ QQQQQQQQQE DQQQQQQQEN 420
PYIYYPTPNP IPVKMPKDEK TFKKRNNKNH PANNSNNANK QANPYLENSI PTKNTSKKNA 480
SSKSNESTAN NHKSHSHSHP HSQSLQQQQQ TYHRSPLLEQ LRNSSSDKNS NSNMSLKDIF 540
GHSLEFCKDQ HGSRFIQREL ATSPASEKEV IFNEIRDDAI ELSNDVFGNY VIQKFFEFGS 600
KIQKNTLVDQ FKGNMKQLSL QMYACRVIQK ALEYIDSNQR IELVLELSDS VLQMIKDQNG 660
NHVIQKAIET IPIEKLPFIL SSLTGHIYHL STHSYGCRVI QRLLEFGSSE DQESILNELK 720
DFIPYLIQDQ YGNYVIQYVL QQDQFTNKEM VDIKQEIIET VANNVVEYSK HKFASNVVEK 780
SILYGSKNQK DLIISKILPR DKNHALNLED DSPMILMIKD QFANYVIQKL VNVSEGEGKK 840
LIVIAIRAYL DKLNKSNSLG NRHLASVEKL AALVENAEV 879 |
Gene Ontology | GO:0032473; C:external side of mitochondrial outer membrane; IDA:SGD. GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell. GO:0003729; F:mRNA binding; IDA:SGD. GO:0009060; P:aerobic respiration; IMP:SGD. GO:0008298; P:intracellular mRNA localization; IMP:SGD. GO:0051646; P:mitochondrion localization; IMP:SGD. GO:0007005; P:mitochondrion organization; IMP:SGD. GO:0000288; P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay; IMP:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |