CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005621
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Calnexin 
Protein Synonyms/Alias
 IP90; Major histocompatibility complex class I antigen-binding protein p88; p90 
Gene Name
 CANX 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
87LSGWILSKAKKDDTDubiquitination[1]
103EIAKYDGKWEVEEMKubiquitination[1]
118ESKLPGDKGLVLMSRubiquitination[1, 2]
137AISAKLNKPFLFDTKacetylation[3]
137AISAKLNKPFLFDTKubiquitination[1]
170AYVKLLSKTPELNLDubiquitination[1, 2]
182NLDQFHDKTPYTIMFubiquitination[2]
199DKCGEDYKLHFIFRHubiquitination[2]
217KTGIYEEKHAKRPDAubiquitination[1]
227KRPDADLKTYFTDKKubiquitination[1, 2]
233LKTYFTDKKTHLYTLubiquitination[2]
234KTYFTDKKTHLYTLIubiquitination[2]
380VIDNPNYKGKWKPPMubiquitination[2]
382DNPNYKGKWKPPMIDubiquitination[2]
398PSYQGIWKPRKIPNPubiquitination[1, 2, 4]
401QGIWKPRKIPNPDFFubiquitination[1, 2, 5]
458ANDGWGLKKAADGAAubiquitination[2, 4]
459NDGWGLKKAADGAAEubiquitination[2]
515QTSGMEYKKTDAPQPubiquitination[1]
525DAPQPDVKEEEEEKEsumoylation[6]
547EEEEGEEKLEEKQKSubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [6] Site-specific identification of SUMO-2 targets in cells reveals an inverted SUMOylation motif and a hydrophobic cluster SUMOylation motif.
 Matic I, Schimmel J, Hendriks IA, van Santen MA, van de Rijke F, van Dam H, Gnad F, Mann M, Vertegaal AC.
 Mol Cell. 2010 Aug 27;39(4):641-52. [PMID: 20797634] 
Functional Description
 Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor- mediated endocytosis at the synapse. 
Sequence Annotation
 REPEAT 278 290 1-1.
 REPEAT 295 307 1-2.
 REPEAT 314 326 1-3.
 REPEAT 333 345 1-4.
 REPEAT 348 358 2-1.
 REPEAT 367 377 2-2.
 REPEAT 381 391 2-3.
 REPEAT 395 405 2-4.
 REGION 276 409 P domain (Extended arm) (By similarity).
 REGION 278 345 4 X approximate repeats.
 REGION 348 405 4 X approximate repeats.
 REGION 503 592 Sufficient to mediate interaction with
 METAL 74 74 Calcium; via carbonyl oxygen (By
 METAL 117 117 Calcium; via carbonyl oxygen (By
 METAL 436 436 Calcium (By similarity).
 BINDING 164 164 Carbohydrate (By similarity).
 BINDING 166 166 Carbohydrate (By similarity).
 BINDING 185 185 Carbohydrate (By similarity).
 BINDING 216 216 Carbohydrate (By similarity).
 MOD_RES 137 137 N6-acetyllysine.
 MOD_RES 554 554 Phosphoserine.
 MOD_RES 562 562 Phosphothreonine.
 MOD_RES 564 564 Phosphoserine; by MAPK3.
 MOD_RES 583 583 Phosphoserine.
 LIPID 502 502 S-palmitoyl cysteine.
 LIPID 503 503 S-palmitoyl cysteine.
 DISULFID 160 194 By similarity.
 DISULFID 360 366 By similarity.  
Keyword
 Acetylation; Calcium; Chaperone; Complete proteome; Direct protein sequencing; Disulfide bond; Endoplasmic reticulum; Lectin; Lipoprotein; Membrane; Metal-binding; Palmitate; Phosphoprotein; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 592 AA 
Protein Sequence
MEGKWLLCML LVLGTAIVEA HDGHDDDVID IEDDLDDVIE EVEDSKPDTT APPSSPKVTY 60
KAPVPTGEVY FADSFDRGTL SGWILSKAKK DDTDDEIAKY DGKWEVEEMK ESKLPGDKGL 120
VLMSRAKHHA ISAKLNKPFL FDTKPLIVQY EVNFQNGIEC GGAYVKLLSK TPELNLDQFH 180
DKTPYTIMFG PDKCGEDYKL HFIFRHKNPK TGIYEEKHAK RPDADLKTYF TDKKTHLYTL 240
ILNPDNSFEI LVDQSVVNSG NLLNDMTPPV NPSREIEDPE DRKPEDWDER PKIPDPEAVK 300
PDDWDEDAPA KIPDEEATKP EGWLDDEPEY VPDPDAEKPE DWDEDMDGEW EAPQIANPRC 360
ESAPGCGVWQ RPVIDNPNYK GKWKPPMIDN PSYQGIWKPR KIPNPDFFED LEPFRMTPFS 420
AIGLELWSMT SDIFFDNFII CADRRIVDDW ANDGWGLKKA ADGAAEPGVV GQMIEAAEER 480
PWLWVVYILT VALPVFLVIL FCCSGKKQTS GMEYKKTDAP QPDVKEEEEE KEEEKDKGDE 540
EEEGEEKLEE KQKSDAEEDG GTVSQEEEDR KPKAEEDEIL NRSPRNRKPR RE 592 
Gene Ontology
 GO:0030424; C:axon; IEA:Compara.
 GO:0032839; C:dendrite cytoplasm; IEA:Compara.
 GO:0043197; C:dendritic spine; IEA:Compara.
 GO:0005788; C:endoplasmic reticulum lumen; TAS:Reactome.
 GO:0071556; C:integral to lumenal side of endoplasmic reticulum membrane; TAS:Reactome.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0005840; C:ribosome; IEA:Compara.
 GO:0005509; F:calcium ion binding; TAS:ProtInc.
 GO:0051082; F:unfolded protein binding; NAS:UniProtKB.
 GO:0007568; P:aging; IEA:Compara.
 GO:0019886; P:antigen processing and presentation of exogenous peptide antigen via MHC class II; TAS:Reactome.
 GO:0002474; P:antigen processing and presentation of peptide antigen via MHC class I; TAS:Reactome.
 GO:0072583; P:clathrin-mediated endocytosis; ISS:UniProtKB.
 GO:0043687; P:post-translational protein modification; TAS:Reactome.
 GO:0006457; P:protein folding; TAS:Reactome.
 GO:0018279; P:protein N-linked glycosylation via asparagine; TAS:Reactome.
 GO:0009306; P:protein secretion; TAS:ProtInc.
 GO:0048488; P:synaptic vesicle endocytosis; ISS:UniProtKB. 
Interpro
 IPR001580; Calret/calnex.
 IPR018124; Calret/calnex_CS.
 IPR009033; Calreticulin/calnexin_P_dom.
 IPR008985; ConA-like_lec_gl_sf.
 IPR013320; ConA-like_subgrp. 
Pfam
 PF00262; Calreticulin 
SMART
  
PROSITE
 PS00803; CALRETICULIN_1
 PS00804; CALRETICULIN_2
 PS00805; CALRETICULIN_REPEAT 
PRINTS
 PR00626; CALRETICULIN.