UniProt Accession

 OCLN_HUMAN; Q16625; B5BU70; D2DU64; D2DU65; D2IGC0; D2IGC1; E2CYV9; Q5U1V4; Q8N6K1 

Genbank Protein ID

 U49184; U53823; FJ786083; FJ786084; AF400630; AF400623; AF400624; AF400625; AF400626; AF400627; AF400628; AF400629; GQ225096; GQ225097; GQ225098; GQ402517; AB451306; AB451437; AC145146; AC147575; BC029886; BK001650 

Genbank Nucleotide ID

 AAC50451.1; AAB00195.1; ACT53743.1; ACT53744.1; AAL47094.1; AAL47094.1; AAL47094.1; AAL47094.1; AAL47094.1; AAL47094.1; AAL47094.1; AAL47094.1; ACT83431.1; ACT83432.1; ACT83433.1; ACZ80515.1; BAG70120.1; BAG70251.1; AAH29886.1; DAA01837.1 

Protein Name

 Occludin 

Protein Synonyms/Alias

  

Gene Name

 OCLN 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
276	RKMDRYDKSNILWDK	ubiquitination	[1, 2, 3, 4, 5]
283	KSNILWDKEHIYDEQ	ubiquitination	[1, 2, 4, 5]
299	PNVEEWVKNVSAGTQ	ubiquitination	[1, 2, 4, 5]
330	MAYSSNGKVNDKRFY	ubiquitination	[2]
343	FYPESSYKSTPVPEV	ubiquitination	[2, 4]
379	GNFETPSKRAPAKGR	ubiquitination	[4]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. 

Sequence Annotation

 DOMAIN 60 269 MARVEL.
 MOD_RES 321 321 Phosphoserine.
 MOD_RES 340 340 Phosphoserine (By similarity).
 MOD_RES 369 369 Phosphoserine.
 MOD_RES 370 370 Phosphoserine.
 MOD_RES 398 398 Phosphotyrosine.
 MOD_RES 402 402 Phosphotyrosine.
 MOD_RES 403 403 Phosphothreonine; by PKC/PRKCH.
 MOD_RES 404 404 Phosphothreonine; by PKC/PRKCH.
 MOD_RES 408 408 Phosphoserine.
 MOD_RES 490 490 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Cell junction; Coiled coil; Complete proteome; Disease mutation; Membrane; Phosphoprotein; Reference proteome; Tight junction; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 522 AA 

Protein Sequence

Gene Ontology

 GO:0016324; C:apical plasma membrane; IEA:Compara.
 GO:0016327; C:apicolateral plasma membrane; IEA:Compara.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0005923; C:tight junction; IDA:BHF-UCL.
 GO:0005198; F:structural molecule activity; IEA:InterPro.
 GO:0045216; P:cell-cell junction organization; IMP:MGI.
 GO:0006921; P:cellular component disassembly involved in execution phase of apoptosis; TAS:Reactome.
 GO:0006461; P:protein complex assembly; TAS:ProtInc. 

Interpro

 IPR008253; Marvel.
 IPR021128; MARVEL-like_dom.
 IPR002958; Occludin.
 IPR010844; Occludin_RNApol2_elong_fac_ELL. 

Pfam

 PF01284; MARVEL
 PF07303; Occludin_ELL 

SMART

  

PROSITE

 PS51225; MARVEL 

PRINTS

 PR01258; OCCLUDIN.