UniProt Accession

 FNR_ECOLI; P0A9E5; P03019 

Genbank Protein ID

 J01608; U00096; AP009048 

Genbank Nucleotide ID

 AAA87981.1; AAC74416.1; BAA14927.1 

Protein Name

 Fumarate and nitrate reduction regulatory protein 

Protein Synonyms/Alias

  

Gene Name

 fnr 

Gene Synonyms/Alias

 nirR; b1334; JW1328 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
60	QKGQTLFKAGDELKS	acetylation	[1]
66	FKAGDELKSLYAIRS	acetylation	[1]
163	DMILLLSKKNAEERL	acetylation	[1]
229	GMLAVKGKYITIENN	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Global transcription factor that controls the expression of over 100 target genes in response to anoxia. It facilitates the adaptation to anaerobic growth conditions by regulating the expression of gene products that are involved in anaerobic energy metabolism. When the terminal electron acceptor, O(2), is no longer available, it represses the synthesis of enzymes involved in aerobic respiration and increases the synthesis of enzymes required for anaerobic respiration. 

Sequence Annotation

 DOMAIN 164 237 HTH crp-type.
 DNA_BIND 197 216 H-T-H motif (By similarity).
 REGION 20 29 Essential for the oxygen-regulated
 REGION 47 50 Activating region 2A (Potential).
 REGION 60 61 Activating region 3A (Potential).
 REGION 71 75 Activating region 1A (Potential).
 REGION 81 81 Activating region 3B (Potential).
 REGION 85 87 Activating region 3C (Potential).
 REGION 112 112 Activating region 3D (Potential).
 REGION 116 121 Activating region 1B (Potential).
 REGION 123 124 Activating region 2B (Potential).
 REGION 127 128 Activating region 2C (Potential).
 REGION 140 159 Dimerization (Potential).
 REGION 181 191 Activating region 1C (Potential).
 METAL 20 20 Iron-sulfur (4Fe-4S) (Potential).
 METAL 23 23 Iron-sulfur (4Fe-4S) (Potential).
 METAL 29 29 Iron-sulfur (4Fe-4S) (Potential).
 METAL 122 122 Iron-sulfur (4Fe-4S) (Potential).  

Keyword

 4Fe-4S; Activator; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; Iron; Iron-sulfur; Metal-binding; Reference proteome; Repressor; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 250 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IDA:EcoCyc.
 GO:0003677; F:DNA binding; IDA:EcoCyc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:EcoCyc.
 GO:0009061; P:anaerobic respiration; IEP:EcoCyc.
 GO:0071731; P:response to nitric oxide; IEP:EcoCyc.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR018490; cNMP-bd-like.
 IPR000595; cNMP-bd_dom.
 IPR012318; HTH_CRP_2.
 IPR014710; RmlC-like_jellyroll.
 IPR001808; Tscrpt_reg_HTH_Crp.
 IPR018335; Tscrpt_reg_HTH_Crp-type_CS.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF00027; cNMP_binding
 PF00325; Crp 

SMART

 SM00100; cNMP
 SM00419; HTH_CRP 

PROSITE

 PS50042; CNMP_BINDING_3
 PS00042; HTH_CRP_1
 PS51063; HTH_CRP_2 

PRINTS

 PR00034; HTHCRP.