CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002541
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Valine--tRNA ligase, mitochondrial 
Protein Synonyms/Alias
 Valyl-tRNA synthetase; ValRS 
Gene Name
 VAS1 
Gene Synonyms/Alias
 YGR094W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
128PIPEFIDKTVPGEKKacetylation[1]
266VGKVWEWKEEYHSRIacetylation[1]
274EEYHSRIKNQIQKLGubiquitination[2]
279RIKNQIQKLGASYDWubiquitination[2]
339SNLEVENKDVKSRTLacetylation[1]
339SNLEVENKDVKSRTLubiquitination[3]
342EVENKDVKSRTLLSVubiquitination[3]
450QNDYNTGKRHNLEFIubiquitination[2]
491KKVIEQLKEKNLYVGacetylation[1]
748EKAKIGQKESYPNGIubiquitination[2]
793GYRKFCNKIYQATKFacetylation[1]
922LPKRSTEKAASIVKAubiquitination[2]
928EKAASIVKASYPVYVubiquitination[2]
968LSEYNILKNGKVFVEacetylation[1]
983SNHEEYFKTAEDQKDacetylation[1]
1069NSKDYETKANTQAKEacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 
Functional Description
  
Sequence Annotation
 MOTIF 190 200 "HIGH" region.
 MOTIF 703 707 "KMSKS" region.
 BINDING 706 706 ATP (By similarity).
 MOD_RES 73 73 Phosphoserine.
 MOD_RES 294 294 Phosphoserine.
 MOD_RES 332 332 Phosphoserine.
 MOD_RES 707 707 Phosphoserine.
 MOD_RES 1003 1003 Phosphothreonine.  
Keyword
 Alternative initiation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Mitochondrion; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1104 AA 
Protein Sequence
MNKWLNTLSK TFTFRLLNCH YRRSLPLCQN FSLKKSLTHN QVRFFKMSDL DNLPPVDPKT 60
GEVIINPLKE DGSPKTPKEI EKEKKKAEKL LKFAAKQAKK NAAATTGASQ KKPKKKKEVE 120
PIPEFIDKTV PGEKKILVSL DDPALKAYNP ANVESSWYDW WIKTGVFEPE FTADGKVKPE 180
GVFCIPAPPP NVTGALHIGH ALTIAIQDSL IRYNRMKGKT VLFLPGFDHA GIATQSVVEK 240
QIWAKDRKTR HDYGREAFVG KVWEWKEEYH SRIKNQIQKL GASYDWSREA FTLSPELTKS 300
VEEAFVRLHD EGVIYRASRL VNWSVKLNTA ISNLEVENKD VKSRTLLSVP GYDEKVEFGV 360
LTSFAYPVIG SDEKLIIATT RPETIFGDTA VAVHPDDDRY KHLHGKFIQH PFLPRKIPII 420
TDKEAVDMEF GTGAVKITPA HDQNDYNTGK RHNLEFINIL TDDGLLNEEC GPEWQGMKRF 480
DARKKVIEQL KEKNLYVGQE DNEMTIPTCS RSGDIIEPLL KPQWWVSQSE MAKDAIKVVR 540
DGQITITPKS SEAEYFHWLG NIQDWCISRQ LWWGHRCPVY FINIEGEEHD RIDGDYWVAG 600
RSMEEAEKKA AAKYPNSKFT LEQDEDVLDT WFSSGLWPFS TLGWPEKTKD METFYPFSML 660
ETGWDILFFW VTRMILLGLK LTGSVPFKEV FCHSLVRDAQ GRKMSKSLGN VIDPLDVITG 720
IKLDDLHAKL LQGNLDPREV EKAKIGQKES YPNGIPQCGT DAMRFALCAY TTGGRDINLD 780
ILRVEGYRKF CNKIYQATKF ALMRLGDDYQ PPATEGLSGN ESLVEKWILH KLTETSKIVN 840
EALDKRDFLT STSSIYEFWY LICDVYIENS KYLIQEGSAI EKKSAKDTLY ILLDNALKLI 900
HPFMPFISEE MWQRLPKRST EKAASIVKAS YPVYVSEYDD VKSANAYDLV LNITKEARSL 960
LSEYNILKNG KVFVESNHEE YFKTAEDQKD SIVSLIKAID EVTVVRDASE IPEGCVLQSV 1020
NPEVNVHLLV KGHVDIDAEI AKVQKKLEKA KKSKNGIEQT INSKDYETKA NTQAKEANKS 1080
KLDNTVAEIE GLEATIENLK RLKL 1104 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004832; F:valine-tRNA ligase activity; IDA:SGD.
 GO:0006450; P:regulation of translational fidelity; IEA:GOC.
 GO:0006438; P:valyl-tRNA aminoacylation; IDA:SGD. 
Interpro
 IPR001412; aa-tRNA-synth_I_CS.
 IPR002300; aa-tRNA-synth_Ia.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR010978; tRNA-bd_arm.
 IPR009080; tRNAsynth_1a_anticodon-bd.
 IPR013155; V/L/I-tRNA-synth_anticodon-bd.
 IPR009008; Val/Leu/Ile-tRNA-synth_edit.
 IPR002303; Valyl-tRNA_ligase. 
Pfam
 PF08264; Anticodon_1
 PF00133; tRNA-synt_1 
SMART
  
PROSITE
 PS00178; AA_TRNA_LIGASE_I 
PRINTS
 PR00986; TRNASYNTHVAL.