UniProt Accession

 WASP_HUMAN; P42768; Q9BU11; Q9UNJ9 

Genbank Protein ID

 U12707; U18935; U19927; AF115549; AF196970; BC002961; BC012738 

Genbank Nucleotide ID

 AAA62663.1; AAA60381.1; AAC50140.1; AAD26691.1; AAH02961.1; AAH12738.1 

Protein Name

 Wiskott-Aldrich syndrome protein 

Protein Synonyms/Alias

 WASp 

Gene Name

 WAS 

Gene Synonyms/Alias

 IMD2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
76	CGAVCFVKDNPQKSY	ubiquitination	[1, 2]
81	FVKDNPQKSYFIRLY	ubiquitination	[2]
144	FRALVQEKIQKRNQR	ubiquitination	[1, 2, 3, 4]
147	LVQEKIQKRNQRQSG	ubiquitination	[2]
235	SGKKKISKADIGAPS	ubiquitination	[2]

Reference

 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Effector protein for Rho-type GTPases. Regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. 

Sequence Annotation

 DOMAIN 39 148 WH1.
 DOMAIN 238 251 CRIB.
 REPEAT 337 346 GRSGPLPPXP motif 1.
 REPEAT 376 385 GRSGPLPPXP motif 2.
 DOMAIN 430 447 WH2.
 MOD_RES 291 291 Phosphotyrosine; by FYN and HCK.
 MOD_RES 483 483 Phosphoserine; by CK2.
 MOD_RES 484 484 Phosphoserine; by CK2.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Disease mutation; Phosphoprotein; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 502 AA 

Protein Sequence

Gene Ontology

 GO:0015629; C:actin cytoskeleton; TAS:ProtInc.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0012506; C:vesicle membrane; IEA:Compara.
 GO:0005083; F:small GTPase regulator activity; TAS:ProtInc.
 GO:0030041; P:actin filament polymerization; IEA:Compara.
 GO:0030048; P:actin filament-based movement; IEA:Compara.
 GO:0008154; P:actin polymerization or depolymerization; TAS:ProtInc.
 GO:0007596; P:blood coagulation; TAS:ProtInc.
 GO:0016197; P:endosomal transport; IEA:Compara.
 GO:0008544; P:epidermis development; TAS:ProtInc.
 GO:0038096; P:Fc-gamma receptor signaling pathway involved in phagocytosis; TAS:Reactome.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0006461; P:protein complex assembly; TAS:ProtInc.
 GO:0042110; P:T cell activation; IEA:Compara.
 GO:0050852; P:T cell receptor signaling pathway; TAS:Reactome. 

Interpro

 IPR000697; EVH1.
 IPR000095; PAK_box_Rho-bd.
 IPR011993; PH_like_dom.
 IPR011026; WASP_C.
 IPR003124; WH2_dom. 

Pfam

 PF00786; PBD
 PF00568; WH1
 PF02205; WH2 

SMART

 SM00285; PBD
 SM00461; WH1
 SM00246; WH2 

PROSITE

 PS50108; CRIB
 PS50229; WH1
 PS51082; WH2 

PRINTS