CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018369
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase MYLIP 
Protein Synonyms/Alias
 Inducible degrader of the LDL-receptor; Idol; Myosin regulatory light chain interacting protein; MIR 
Gene Name
 MYLIP 
Gene Synonyms/Alias
 BZF1; IDOL; BM-023; PP5242 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
137DYNQNTAKYNYEELCubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol- dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR. 
Sequence Annotation
 DOMAIN 1 279 FERM.
 ZN_FING 387 422 RING-type.
 REGION 431 433 Critical for homodimerization.
 METAL 360 360 Iron.
 METAL 363 363 Iron.
 METAL 368 368 Iron.  
Keyword
 3D-structure; Alternative splicing; Cell membrane; Complete proteome; Cytoplasm; Iron; Ligase; Membrane; Metal-binding; Polymorphism; Reference proteome; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 445 AA 
Protein Sequence
MLCYVTRPDA VLMEVEVEAK ANGEDCLNQV CRRLGIIEVD YFGLQFTGSK GESLWLNLRN 60
RISQQMDGLA PYRLKLRVKF FVEPHLILQE QTRHIFFLHI KEALLAGHLL CSPEQAVELS 120
ALLAQTKFGD YNQNTAKYNY EELCAKELSS ATLNSIVAKH KELEGTSQAS AEYQVLQIVS 180
AMENYGIEWH SVRDSEGQKL LIGVGPEGIS ICKDDFSPIN RIAYPVVQMA TQSGKNVYLT 240
VTKESGNSIV LLFKMISTRA ASGLYRAITE THAFYRCDTV TSAVMMQYSR DLKGHLASLF 300
LNENINLGKK YVFDIKRTSK EVYDHARRAL YNAGVVDLVS RNNQSPSHSP LKSSESSMNC 360
SSCEGLSCQQ TRVLQEKLRK LKEAMLCMVC CEEEINSTFC PCGHTVCCES CAAQLQSCPV 420
CRSRVEHVQH VYLPTHTSLL NLTVI 445 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005856; C:cytoskeleton; IEA:InterPro.
 GO:0019898; C:extrinsic to membrane; IEA:InterPro.
 GO:0005622; C:intracellular; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0008092; F:cytoskeletal protein binding; IDA:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006928; P:cellular component movement; IMP:UniProtKB.
 GO:0042632; P:cholesterol homeostasis; IEA:Compara.
 GO:0010989; P:negative regulation of low-density lipoprotein particle clearance; IEA:Compara.
 GO:0007399; P:nervous system development; IMP:UniProtKB.
 GO:0045732; P:positive regulation of protein catabolic process; IEA:Compara.
 GO:0031648; P:protein destabilization; IEA:Compara.
 GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IEA:Compara.
 GO:0032803; P:regulation of low-density lipoprotein particle receptor catabolic process; IEA:Compara. 
Interpro
 IPR019749; Band_41_domain.
 IPR019750; Band_41_fam.
 IPR000798; Ez/rad/moesin_like.
 IPR014352; FERM/acyl-CoA-bd_prot_3-hlx.
 IPR019748; FERM_central.
 IPR000299; FERM_domain.
 IPR018979; FERM_N.
 IPR011993; PH_like_dom.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF00373; FERM_M
 PF09379; FERM_N 
SMART
 SM00295; B41
 SM00184; RING 
PROSITE
 PS00660; FERM_1
 PS00661; FERM_2
 PS50057; FERM_3
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS
 PR00935; BAND41.
 PR00661; ERMFAMILY.