CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030690
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Spermine synthase 
Protein Synonyms/Alias
 cDNA FLJ50114, highly similar to Spermine synthase (EC 2.5.1.22) 
Gene Name
 SMS 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
2******MKELSQDSTubiquitination[1]
55DSPYQNIKILHSKQFubiquitination[1, 2, 3, 4, 5]
90RAIMGSGKEDYTGKDubiquitination[3]
134QMVIDGCKKYMRKTCubiquitination[3]
149GDVLDNLKGDCYQVLubiquitination[4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 275 AA 
Protein Sequence
MKELSQDSTG RVKRLPPIVR GGAIDRYWPT ADGRLVEYDI DEVVYDEDSP YQNIKILHSK 60
QFGNILILSG DVNLAESDLA YTRAIMGSGK EDYTGKDVLI LGGGDGGILC EIVKLKPKMV 120
TMVEIDQMVI DGCKKYMRKT CGDVLDNLKG DCYQVLIEDC IPVLKRYAKE GREFDYVIND 180
LTAVPISTSP EEDSTWEFLR LILDLSMKVL KQDGKYFTQG NCVNLTEALS LYEEQLGRLY 240
CPVEFSKEIV CVPSYLELFL LQFWRVRSSK CALEG 275 
Gene Ontology
 GO:0016768; F:spermine synthase activity; IEA:InterPro.
 GO:0006597; P:spermine biosynthetic process; IEA:InterPro. 
Interpro
 IPR001045; Spermidine/spermine_synthase.
 IPR015576; Spermine_synthase. 
Pfam
 PF01564; Spermine_synth 
SMART
  
PROSITE
 PS01330; SPERMIDINE_SYNTHASE_1
 PS51006; SPERMIDINE_SYNTHASE_2 
PRINTS