CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012207
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nucleolar GTP-binding protein 2 
Protein Synonyms/Alias
 Autoantigen NGP-1 
Gene Name
 GNL2 
Gene Synonyms/Alias
 NGP1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
58NSRGKIIKPLQYQSTubiquitination[1]
79ARVEPNIKWFGNTRVubiquitination[2]
199EAQEEIYKKGQSKRIubiquitination[3]
323IGYPNVGKSSVINTLubiquitination[4]
388VVQVEKIKSPEDHIGubiquitination[4]
411EYISKTYKIDSWENAubiquitination[4]
434FRTGKLLKGGEPDLQubiquitination[4]
603VIKALDEKIAKYQKFacetylation[2]
705YYETHNVKNRNRNKKubiquitination[4]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation (By similarity). 
Sequence Annotation
 DOMAIN 322 377 G.
 NP_BIND 317 324 GTP (Potential).
 NP_BIND 361 365 GTP (Potential).  
Keyword
 Complete proteome; GTP-binding; Nucleotide-binding; Nucleus; Polymorphism; Reference proteome; Ribosome biogenesis. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 731 AA 
Protein Sequence
MVKPKYKGRS TINPSKASTN PDRVQGAGGQ NMRDRATIRR LNMYRQKERR NSRGKIIKPL 60
QYQSTVASGT VARVEPNIKW FGNTRVIKQS SLQKFQEEMD TVMKDPYKVV MKQSKLPMSL 120
LHDRIRPHNL KVHILDTESF ETTFGPKSQR KRPNLFASDM QSLIENAEMS TESYDQGKDR 180
DLVTEDTGVR NEAQEEIYKK GQSKRIWGEL YKVIDSSDVV VQVLDARDPM GTRSPHIETY 240
LKKEKPWKHL IFVLNKCDLV PTWATKRWVA VLSQDYPTLA FHASLTNPFG KGAFIQLLRQ 300
FGKLHTDKKQ ISVGFIGYPN VGKSSVINTL RSKKVCNVAP IAGETKVWQY ITLMRRIFLI 360
DCPGVVYPSE DSETDIVLKG VVQVEKIKSP EDHIGAVLER AKPEYISKTY KIDSWENAED 420
FLEKLAFRTG KLLKGGEPDL QTVGKMVLND WQRGRIPFFV KPPNAEPLVA PQLLPSSSLE 480
VVPEAAQNNP GEEVTETAGE GSESIIKEET EENSHCDANT EMQQILTRVR QNFGKINVVP 540
QFSGDDLVPV EVSDLEEELE SFSDEEEEEQ EQQRDDAEES SSEPEEENVG NDTKAVIKAL 600
DEKIAKYQKF LDKAKAKKFS AVRISKGLSE KIFAKPEEQR KTLEEDVDDR APSKKGKKRK 660
AQREEEQEHS NKAPRALTSK ERRRAVRQQR PKKVGVRYYE THNVKNRNRN KKKTNDSEGQ 720
KHKRKKFRQK Q 731 
Gene Ontology
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0005525; F:GTP binding; NAS:UniProtKB.
 GO:0003924; F:GTPase activity; NAS:UniProtKB.
 GO:0042254; P:ribosome biogenesis; IBA:RefGenome. 
Interpro
 IPR023179; GTP-bd_ortho_bundle.
 IPR006073; GTP_binding_domain.
 IPR024929; NOG2.
 IPR012971; NOG2_N_dom.
 IPR027417; P-loop_NTPase. 
Pfam
 PF01926; MMR_HSR1
 PF08153; NGP1NT 
SMART
  
PROSITE
  
PRINTS
 PR00326; GTP1OBG.