UniProt Accession

 EHMT1_HUMAN; Q9H9B1; B1AQ58; B1AQ59; Q86X08; Q8TCN7; Q96F53; Q96JF1; Q96KH4 

Genbank Protein ID

 AK022941; AL590627; AL611925; AL590627; AL611925; AL611925; AL590627; AL611925; AL590627; BC011608; BC047504; AY083210; AB028932; AB058779; AL713772 

Genbank Nucleotide ID

 BAB14321.1; CAI17354.1; CAI17354.1; CAI17355.1; CAI17355.1; CAH71076.1; CAH71076.1; CAH71077.1; CAH71077.1; AAH11608.2; AAH47504.1; AAM09024.1; BAB56104.1; BAB47505.2; CAD28534.1 

Protein Name

 Histone-lysine N-methyltransferase EHMT1 

Protein Synonyms/Alias

 Euchromatic histone-lysine N-methyltransferase 1; Eu-HMTase1; G9a-like protein 1; GLP; GLP1; Histone H3-K9 methyltransferase 5; H3-K9-HMTase 5; Lysine N-methyltransferase 1D 

Gene Name

 EHMT1 

Gene Synonyms/Alias

 EUHMTASE1; GLP; KIAA1876; KMT1D 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
827	EAVKYLIKAGALVDP	ubiquitination	[1]

Reference

 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non- histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. 

Sequence Annotation

 REPEAT 737 766 ANK 1.
 REPEAT 772 801 ANK 2.
 REPEAT 805 834 ANK 3.
 REPEAT 838 868 ANK 4.
 REPEAT 872 901 ANK 5.
 REPEAT 905 934 ANK 6.
 REPEAT 938 967 ANK 7.
 REPEAT 971 1004 ANK 8.
 DOMAIN 1060 1123 Pre-SET.
 DOMAIN 1126 1243 SET.
 REGION 905 907 Histone H3K9me binding.
 REGION 1136 1138 S-adenosyl-L-methionine binding.
 REGION 1162 1181 Interaction with histone H3.
 REGION 1200 1201 S-adenosyl-L-methionine binding.
 REGION 1242 1245 Interaction with histone H3.
 METAL 1062 1062 Zinc 1.
 METAL 1062 1062 Zinc 2.
 METAL 1064 1064 Zinc 1.
 METAL 1068 1068 Zinc 1.
 METAL 1068 1068 Zinc 3.
 METAL 1073 1073 Zinc 1.
 METAL 1075 1075 Zinc 2.
 METAL 1105 1105 Zinc 2.
 METAL 1105 1105 Zinc 3.
 METAL 1109 1109 Zinc 2.
 METAL 1111 1111 Zinc 3.
 METAL 1115 1115 Zinc 3.
 METAL 1203 1203 Zinc 4.
 METAL 1256 1256 Zinc 4.
 METAL 1258 1258 Zinc 4.
 METAL 1263 1263 Zinc 4.
 BINDING 1155 1155 Histone H3K9me.
 BINDING 1173 1173 S-adenosyl-L-methionine.
 BINDING 1257 1257 S-adenosyl-L-methionine; via amide  

Keyword

 3D-structure; Alternative splicing; ANK repeat; Chromatin regulator; Chromosome; Complete proteome; Disease mutation; Mental retardation; Metal-binding; Methyltransferase; Nucleus; Polymorphism; Reference proteome; Repeat; S-adenosyl-L-methionine; Transferase; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1298 AA 

Protein Sequence

Gene Ontology

 GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0046976; F:histone methyltransferase activity (H3-K27 specific); ISS:UniProtKB.
 GO:0046974; F:histone methyltransferase activity (H3-K9 specific); ISS:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006306; P:DNA methylation; ISS:UniProtKB.
 GO:0009790; P:embryo development; ISS:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; ISS:UniProtKB.
 GO:0018027; P:peptidyl-lysine dimethylation; IDA:UniProtKB.
 GO:0018026; P:peptidyl-lysine monomethylation; ISS:UniProtKB. 

Interpro

 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR007728; Pre-SET_dom.
 IPR003606; Pre-SET_Zn-bd_sub.
 IPR001214; SET_dom. 

Pfam

 PF00023; Ank
 PF12796; Ank_2
 PF05033; Pre-SET
 PF00856; SET 

SMART

 SM00248; ANK
 SM00468; PreSET
 SM00317; SET 

PROSITE

 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS50867; PRE_SET
 PS50280; SET 

PRINTS

 PR01415; ANKYRIN.