| Tag | Content |
|---|
| CPLM ID | CPLM-016004 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | E3 ubiquitin-protein ligase MIB1 |
Protein Synonyms/Alias | DAPK-interacting protein 1; DIP-1; Mind bomb homolog 1 |
Gene Name | Mib1 |
Gene Synonyms/Alias | Dip1; Kiaa1323; Mib |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 28 | GPDWKWGKQDGGEGH | ubiquitination | [1, 2] | | 222 | LKCVQDAKGGSFYRD | ubiquitination | [1] | | 772 | DLSIRNKKGQSPLDL | ubiquitination | [2] | | 787 | CPDPSLCKALAKCHK | ubiquitination | [2] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] [2] BTB-ZF factors recruit the E3 ligase cullin 3 to regulate lymphoid effector programs. Mathew R, Seiler MP, Scanlon ST, Mao AP, Constantinides MG, Bertozzi-Villa C, Singer JD, Bendelac A. Nature. 2012 Nov 22;491(7425):618-21. [ PMID: 23086144] |
Functional Description | E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation (By similarity). Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. |
Sequence Annotation | DOMAIN 6 74 MIB/HERC2 1.
DOMAIN 143 221 MIB/HERC2 2.
REPEAT 430 460 ANK 1.
REPEAT 463 492 ANK 2.
REPEAT 496 525 ANK 3.
REPEAT 529 558 ANK 4.
REPEAT 562 591 ANK 5.
REPEAT 595 627 ANK 6.
REPEAT 631 661 ANK 7.
REPEAT 665 694 ANK 8.
REPEAT 698 729 ANK 9.
ZN_FING 79 126 ZZ-type.
ZN_FING 819 854 RING-type 1.
ZN_FING 866 901 RING-type 2.
ZN_FING 963 996 RING-type 3. |
Keyword | ANK repeat; Cell membrane; Coiled coil; Complete proteome; Cytoplasm; Ligase; Membrane; Metal-binding; Notch signaling pathway; Reference proteome; Repeat; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1006 AA |
Protein Sequence | MSNSRNNRVM VEGVGARVVR GPDWKWGKQD GGEGHVGTVR SFESPEEVVV VWDNGTAANY 60
RCSGAYDLRI LDSAPTGIKH DGTMCDTCRQ QPIIGIRWKC AECTNYDLCT VCYHGDKHHL 120
RHRFYRITTP GSERVLLESR RKSKKITARG IFAGARVVRG VDWQWEDQDG GNGRRGKVTE 180
IQDWSASSPH SAAYVLWDNG AKNLYRVGFE GMSDLKCVQD AKGGSFYRDH CPVLGEQNGN 240
RNPGGLQIGD LVNIDLDLEI VQSLQHGHGG WTDGMFETLT TTGTVCGIDE DHDIVVQYPS 300
GNRWTFNPAV LTKANIVRSG DAAQGAEGGT SQFQVGDLVQ VCYDLERIKL LQRGHGEWAE 360
AMLPTLGKVG RVQQIYSDSD LKVEVCGTSW TYNPAAVSKV APAGSAISNA SGERLSQLLK 420
KLFETQESGD LNEELVKAAA NGDVAKVEDL LKRPDVDVNG QCAGHTAMQA ASQNGHVDIL 480
KLLLKQNVDV EAEDKDGDRA VHHAAFGDEG AVIEVLHRGS ADLNARNKRR QTPLHIAVNK 540
GHLQVVKTLL DFGCHPSLQD SEGDTPLHDA ISKKRDDILA VLLEAGADVT ITNNNGFNAL 600
HHAALRGNPS AMRVLLSKLP RPWIVDEKKD DGYTALHLAA LNNHVEVAEL LVHQGNANLD 660
IQNVNQQTAL HLAVERQHTQ IVRLLVRAGA KLDIQDKDGD TPLHEALRHH TLSQLRQLQD 720
MQDVGKVDAA WEPSKNTLIM GLGTQGAEKK SAASIACFLA ANGADLSIRN KKGQSPLDLC 780
PDPSLCKALA KCHKEKVSGQ VGSRSPSMIS NDSETLEECM VCSDMKRDTL FGPCGHIATC 840
SLCSPRVKKC LICKEQVQSR TKIEECVVCS DKKAAVLFQP CGHMCACENC ASLMKKCVQC 900
RAVVERRVPF ITCCGGKSSE DPSDEISSGN IPVLQKDKDN TNVNADVQKL QQQLQDIKEQ 960
TMCPVCLDRL KNMIFLCGHG TCQLCGDRMS ECPICRKAIE RRILLY 1006 |
Gene Ontology | |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |