CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044635
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ATP-dependent (S)-NAD(P)H-hydrate dehydratase 
Protein Synonyms/Alias
 ATP-dependent NAD(P)HX dehydratase; Carbohydrate kinase domain-containing protein 
Gene Name
 Carkd 
Gene Synonyms/Alias
 0710008K08Rik; mCG_17317 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
47IVPALTSKKHKGQDGacetylation[1]
Reference
 [1] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753] 
Functional Description
 Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity). 
Sequence Annotation
 DOMAIN 33 324 YjeF C-terminal (By similarity).
 NP_BIND 226 230 ATP (By similarity).
 NP_BIND 245 254 ATP (By similarity).
 REGION 186 192 NAD(P)HX (By similarity).
 BINDING 133 133 NAD(P)HX; via amide nitrogen (By
 BINDING 255 255 NAD(P)HX (By similarity).  
Keyword
 ATP-binding; Complete proteome; Lyase; Mitochondrion; NAD; Nucleotide-binding; Phosphoprotein; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 327 AA 
Protein Sequence
MGVRCVAIRA CGGVLQRALS LHTAHATKDM ENLFQLVRNI VPALTSKKHK GQDGRIGIVG 60
GCQEYTGAPY FAGISALKVG ADLTHVFCAR EAAPVIKSYS PELIVHPVLD SSNAVEEVEK 120
WLPRLHALVV GPGLGRDDLL LNNVRGILES TKARDIPVVI DADGLWLVAQ QPALIHSYHK 180
AILTPNHVEF SRLWEAVLSS PMDSNDLKGS TLKLSQALGN ITVVQKGEQD LISNGQQVLV 240
CNQEGSSRRC GGQGDLLSGS LGVMVHWALR AGPEKTNGSS PLLVAAWGAC TLTRECNRQA 300
FQKYGRSTTT TDMITEVGTA FSRLFTT 327 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0047453; F:ATP-dependent NAD(P)H-hydrate dehydratase activity; IEA:HAMAP.
 GO:0046496; P:nicotinamide nucleotide metabolic process; IEA:HAMAP. 
Interpro
 IPR026599; NnrD/CARKD.
 IPR000631; YjeF_C_carb_kinase-rel. 
Pfam
 PF01256; Carb_kinase 
SMART
  
PROSITE
 PS51383; YJEF_C_3 
PRINTS