CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004137
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Myosin-8 
Protein Synonyms/Alias
 Myosin heavy chain 8; Myosin heavy chain, skeletal muscle, perinatal; MyHC-perinatal 
Gene Name
 Myh8 
Gene Synonyms/Alias
 Myhsp 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
24PYLRKSEKERIEAQNubiquitination[1]
32ERIEAQNKPFDAKTSubiquitination[1]
46SVFVAEPKESYVKSVubiquitination[1]
237LEAFGNAKTVRNDNSubiquitination[1]
726GDFKQRYKVLNASAIubiquitination[1]
915CEQLIKNKIQLEAKIubiquitination[1]
921NKIQLEAKIKEVTERubiquitination[1]
979HATENKVKNLTEEMAubiquitination[1]
994GLDENIAKLTKEKKAubiquitination[1]
997ENIAKLTKEKKALQEubiquitination[1]
1027VNTLTKAKTKLEQQVubiquitination[1]
1029TLTKAKTKLEQQVDDubiquitination[1]
1045EGSLEQEKKLRMDLEubiquitination[1]
1076TMDIENDKQQLDEKLubiquitination[1]
1082DKQQLDEKLKKKEFEubiquitination[1]
1506ETLRRENKNLQQEISubiquitination[1]
1524EQIAEGGKHIHELEKubiquitination[1]
1531KHIHELEKIKKQVEQubiquitination[1]
1619NDALRVKKKMEGDLNubiquitination[1]
1787AHLERMKKNMEQTVKubiquitination[1]
1794KNMEQTVKDLQHRLDubiquitination[1]
1834GEVENEQKRNAEAVKubiquitination[1]
1862YQTEEDRKNVLRLQDubiquitination[1]
1901ANLAKFRKLQHELEEubiquitination[1]
1922IAESQVNKLRVKSREubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Muscle contraction. 
Sequence Annotation
 DOMAIN 1 780 Myosin head-like.
 DOMAIN 781 813 IQ.
 NP_BIND 181 188 ATP.
 REGION 658 680 Actin-binding.
 REGION 760 774 Actin-binding.
 MOD_RES 132 132 N6,N6,N6-trimethyllysine (Potential).
 MOD_RES 951 951 Phosphoserine.
 MOD_RES 1202 1202 Phosphoserine.  
Keyword
 Actin-binding; ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Methylation; Motor protein; Muscle protein; Myosin; Nucleotide-binding; Phosphoprotein; Reference proteome; Thick filament. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1937 AA 
Protein Sequence
MSAGSDAEMA IFGEAAPYLR KSEKERIEAQ NKPFDAKTSV FVAEPKESYV KSVIQSKDGG 60
KVTVKTESGA TLTVKEDQVF PMNPPKYDKI EDMAMMTHLH EPGVLYNLKE RYAAWMIYTY 120
SGLFCVTVNP YKWLPVYNPE VVAAYRGKKR QEAPPHIFSI SDNAYQFMLT DRENQSILIT 180
GESGAGKTVN TKRVIQYFAT IAVTGDKKKE ESGKMQGTLE DQIISANPLL EAFGNAKTVR 240
NDNSSRFGKF IRIHFGTTGK LASADIETYL LEKSRVTFQL KAERSYHIFY QITSNKKPEL 300
IEMLLITTNP YDYAFVSQGE ITVPSIDDQE ELMATDSAID ILGFSPEEKV SIYKLTGAVM 360
HYGNMKFKQK QREEQAEPDG TEVADKAAYL QCLNSADLLK ALCYPRVKVG NEYVTKGQTV 420
QQVYNAVGAL AKAVYEKMFL WMVTRINQQL DTKQPRQYFI GVLDIAGFEI FDFNSLEQLC 480
INFTNEKLQQ FFNHHMFVLE QEEYKKEGIE WTFIDFGMDL AACIELIEKP LGIFSILEEE 540
CMFPKATDTS FKNKLYDQHL GKSNNFQKPK PTKGKAEAHF SLVHYAGTVD YNITGWLDKN 600
KDPLNDTVVG LYQKSAMKTL ASLFSTYASA EADGGAKKGA KKKGSSFQTV SALFRENLNK 660
LMTNLRSTHP HFVRCIIPNE TKTPGAMEHE LVLHQLRCNG VLEGIRICRK GFPSRILYGD 720
FKQRYKVLNA SAIPEGQFID SKKASEKLLG SIDIDHTQYK FGHTKVFFKA GLLGLLEEMR 780
DEKLAQIITR TQAVCRGYLM RVEYQKMLLR RESIFCIQYN VRAFMNVKHW PWMKLFFKIK 840
PLLKSAETEK EMATMKEEFQ KTKDELAKSE AKRKELEEKM VTLLKEKNDL QLQVQSEADS 900
LADAEERCEQ LIKNKIQLEA KIKEVTERAE DEEEINAELT AKKRKLEDEC SELKKDIDDL 960
ELTLAKVEKE KHATENKVKN LTEEMAGLDE NIAKLTKEKK ALQEAHQQTL DDLQAEEDKV 1020
NTLTKAKTKL EQQVDDLEGS LEQEKKLRMD LERAKRKLEG DLKLAQESTM DIENDKQQLD 1080
EKLKKKEFEI SNLISKIEDE QAVEIQLQKK IKELQARIEE LEEEIEAERA SRAKAEKQRS 1140
DLSRELEEIS ERLEEAGGAT SAQVEMNKKR ETEFQKLRRD LEEATLQHEA TAAALRKKHA 1200
DSVAELGEQI DNLQRVKQKL EKEKSELKME IDDLSSNAEA IAKAKGNLEK MCRTLEDQVS 1260
ELKSKEEEQQ RLINELTAQR ARLQTEAGEY SRQLDEKDAL VSQLSRSKQA STQQIEELKR 1320
QLEEETKAKN ALAHALQSSR HDCDLLREQY EEEQEGKAEL QRALSKANSE VAQWRTKYET 1380
DAIQRTEELE EAKKKLAQRL QAAEEHVEAV NAKCASLEKT KQRLQNEVED LMIDVERTNA 1440
ACAALDKKQR NFDKVLSEWR QKYEETQAEL ESCQKESRTL STELFKVKNA YEESLDHLET 1500
LRRENKNLQQ EISDLTEQIA EGGKHIHELE KIKKQVEQEK CEIQAALEEA EASLEHEEGK 1560
ILRIQLELNQ VKSEIDRKIA EKDEEIDQLK RNHVRVVETM QSTLDAEIRS RNDALRVKKK 1620
MEGDLNEMEI QLNHANRLAA ESLRNYRNTQ GILKDTQLHL DDALRGQEDL KEQLAIVERR 1680
ANLLQAEIEE LRATLEQTER SRKIAEQELL DASERVQLLH TQNTSLINTK KKLENDVSQL 1740
QSEVEEVIQE SRNAEEKAKK AITDAAMMAE ELKKEQDTSA HLERMKKNME QTVKDLQHRL 1800
DEAEQLALKG GKKQIQKLEA RVRELEGEVE NEQKRNAEAV KGLRKHERRV KELTYQTEED 1860
RKNVLRLQDL VDKLQAKVKS YKRQAEEAEE QSNANLAKFR KLQHELEEAE ERADIAESQV 1920
NKLRVKSREV HTKISAE 1937 
Gene Ontology
 GO:0005925; C:focal adhesion; IEA:Compara.
 GO:0030016; C:myofibril; IEA:UniProtKB-SubCell.
 GO:0016459; C:myosin complex; IDA:MGI.
 GO:0032982; C:myosin filament; IEA:UniProtKB-KW.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003774; F:motor activity; IEA:InterPro. 
Interpro
 IPR000048; IQ_motif_EF-hand-BS.
 IPR027401; Myosin-like_IQ_dom.
 IPR015650; Myosin_1/23/4/7/8/13/15.
 IPR001609; Myosin_head_motor_dom.
 IPR004009; Myosin_N.
 IPR002928; Myosin_tail.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00063; Myosin_head
 PF02736; Myosin_N
 PF01576; Myosin_tail_1 
SMART
 SM00015; IQ
 SM00242; MYSc 
PROSITE
 PS50096; IQ 
PRINTS
 PR00193; MYOSINHEAVY.