CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015088
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA helicase B 
Protein Synonyms/Alias
  
Gene Name
 Helb 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
711VKTLLQGKDFCSFESubiquitination[1]
754LIKDHEKKLIFAVGDacetylation[2]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
 Unwinds duplex DNA with 5'-3' polarity. Has single- strand DNA-dependent ATPase and DNA helicase activities. Prefers ATP and dATP as substrates. During S phase, may facilitate cellular recovery from replication stress. 
Sequence Annotation
 MOD_RES 942 942 Phosphoserine.
 MOD_RES 946 946 Phosphoserine.
 MOD_RES 992 992 Phosphothreonine (By similarity).  
Keyword
 ATP-binding; Complete proteome; Direct protein sequencing; Helicase; Hydrolase; Nucleotide-binding; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1074 AA 
Protein Sequence
MARQDRLREL LGPLHPYKSD DEEEDCAQEE EGEQEEEFVD AEELCSGGIK AGSLPGRARV 60
SIPDEYTKEK CTVYGRFPLK GPWWRVKVQV LKPQRSRSYQ VQGFPAYFLQ VDMSPPDQKQ 120
ICSLFLKECN LASERIQEFL KWVEKVSSFE NLHFENLWET LRLFYRETEK KDKKLSTPRE 180
QQGEEMRVEK SFAFISAMVA LQFPKVMEFL PSLFPRHFKR LISSSSDWVL GCIEDVLGTQ 240
PWKLGFRRIT YREMKLVRCE ASWTAFSQCP SLLQLMTPLQ KNALVIYSKL RQTCREDGHT 300
YIEVKDLTSG LSEHMSFEEA CQSLAFLKDI DVVIYEKDYV FLSELYEAEQ DIASSICELM 360
SRPPWHLKVD VKNVLASIRG AKPNDPGSAE AVEGSKPEEV GSEQGDSVLD AQDGDDHVRS 420
NGEHVANAEI NDVPLDQDQV VALETICANA VTVLSGKGGC GKTTIVSRLF KHMEHLEETE 480
VQQACEDFEQ DQEASEEWLD CPKQSPAGVD KAVEVLLTAP TGKAAGLLRQ RTDLPAYTLC 540
QVNYSFYMWK TKNEVDKPWK FSTVRVLVVD EGSLVSVGIF KSVLQLLCKH SKLSKLIILG 600
DVRQLPSIEP GNMLQDVFET LKSRQCAIEL KTNHRTESQL IVDNATRISR RQFPKFDAEL 660
NICGNPTLPL SIQDKTFIFV RLPEEDSRSQ SSKGEHRSNL YTAVKTLLQG KDFCSFESSK 720
TSQFIAFRRQ DCDLINDCCC KHYTGHLIKD HEKKLIFAVG DKICCTRNAY LSDLLPDKDQ 780
EAEGKGYGDA PDDDAKIKQD FESSTRLCNG EIFFITRDVT DVTFKRKRLL TINNEAGLEV 840
TVDFSKLMAN CQIKHAWART IHTFQGSEEN TVVYVVGKAG RQHWQHVYTA VTRGRSRVYI 900
IAQESELRSA TRKRGFPRQT RLKHFLQKKL SGSCAPSTGF ASQPSSPRVG GRPDTQPPAS 960
HLCRTPDNKA TADSARGDER WLSASVNDDV DTDEESAQLR GSKRIGDGFP FDEESPSKFR 1020
MVEAPSPQVS SVFQNMRLNT LTPRQLFKPT DNQDTGTAGV ADDANDPSNQ EMEM 1074 
Gene Ontology
 GO:0005658; C:alpha DNA polymerase:primase complex; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0043141; F:ATP-dependent 5'-3' DNA helicase activity; IEA:Compara.
 GO:0004003; F:ATP-dependent DNA helicase activity; IDA:MGI.
 GO:0017116; F:single-stranded DNA-dependent ATP-dependent DNA helicase activity; IEA:Compara.
 GO:0006269; P:DNA replication, synthesis of RNA primer; TAS:MGI. 
Interpro
 IPR027044; Helb.
 IPR027417; P-loop_NTPase. 
Pfam
  
SMART
  
PROSITE
  
PRINTS