CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011051
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein EAR1 
Protein Synonyms/Alias
 Endosomal adapter of RSP5 
Gene Name
 EAR1 
Gene Synonyms/Alias
 YMR171C; YM8010.01C; YM8520.20C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
128DADEVRDKLAQLSPEubiquitination[1]
141PEEQFYYKQGEEYIKubiquitination[1]
313TLFPQCEKGDIVGIGubiquitination[1]
384YIEANVKKWGYAKSNubiquitination[2]
496EHHSKNDKSAISGRIubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Systematic approach for validating the ubiquitinated proteome.
 Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
 Anal Chem. 2008 Jun 1;80(11):4161-9. [PMID: 18433149] 
Functional Description
 Components of the endosome-vacuole trafficking pathway that regulates nutrient transport. May be involved in processes which determine whether plasma membrane proteins are degraded or routed to the plasma membrane (By similarity). 
Sequence Annotation
 DOMAIN 172 375 B30.2/SPRY.
 MOD_RES 519 519 Phosphoserine.
 MOD_RES 538 538 Phosphoserine.
 CARBOHYD 110 110 N-linked (GlcNAc...) (Potential).
 CARBOHYD 203 203 N-linked (GlcNAc...) (Potential).
 CARBOHYD 210 210 N-linked (GlcNAc...) (Potential).
 CARBOHYD 256 256 N-linked (GlcNAc...) (Potential).
 CARBOHYD 295 295 N-linked (GlcNAc...) (Potential).  
Keyword
 Complete proteome; Endosome; Glycoprotein; Membrane; Phosphoprotein; Protein transport; Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix; Transport; Vacuole. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 550 AA 
Protein Sequence
MSFKFLIESL LLGSISGQIR CGRSSVIPRG DVSYGGDDTD ELNMDIMLFA FGTLIVVYIV 60
ICIVYFFTKQ IATRLITAYY NEHGPGQRIS LFSDYDENNA HVHSRRLMEN MSLRWPNNLD 120
DADEVRDKLA QLSPEEQFYY KQGEEYIKQN PPFLLNQGLL QQSEDSNPDT TREDPIMNEQ 180
TRQYIQEEGA YAWEFSPNPD MPNHTVIVEN KTEVSFLNYN YDASISTNLP IPCINKVYYC 240
EFKIFETDGP LNSDENVSKG VISFGLSTQP YPYFRLPGRH HHSIAYDSNG ARRFNDSFKL 300
NEQLRTLFPQ CEKGDIVGIG YRSRSGTVFF TRNGKKLNEK SVGGHIRGWK FQYLYPIIGS 360
NVPCQIHVNF GTYGFVYIEA NVKKWGYAKS NGIKLPPPSY EDYGKDTLLE SGGEDNDFDE 420
DFSDGDSDNI AAGSTTNLND DIIIRNGEIL PPPPGFEFTM SPPTGKKIIN EEINLDSLPM 480
LPPSYSDDEH HSKNDKSAIS GRIIGTSRNL ITDEASFDSV DNDNEDENDH ERDPEQFSEF 540
DDYESRMHGI 550 
Gene Ontology
 GO:0005768; C:endosome; IDA:SGD.
 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
 GO:0043328; P:protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IGI:SGD. 
Interpro
 IPR001870; B30.2/SPRY.
 IPR008985; ConA-like_lec_gl_sf.
 IPR018355; SPla/RYanodine_receptor_subgr.
 IPR003877; SPRY_rcpt. 
Pfam
 PF00622; SPRY 
SMART
 SM00449; SPRY 
PROSITE
 PS50188; B302_SPRY 
PRINTS