CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012403
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 AT-rich interactive domain-containing protein 5B 
Protein Synonyms/Alias
 ARID domain-containing protein 5B; MRF1-like protein; Modulator recognition factor 2; MRF-2 
Gene Name
 ARID5B 
Gene Synonyms/Alias
 DESRT; MRF2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
32FQFHLEGKPRILSLGubiquitination[1, 2]
74RQLLSSSKLYFLPEDubiquitination[1, 2, 3]
111VKLEDLVKWVHSDFSubiquitination[1]
119WVHSDFSKWRCGFHAubiquitination[1]
130GFHAGPVKTEALGRNubiquitination[1]
140ALGRNGQKEALLKYRubiquitination[1]
145GQKEALLKYRQSTLNubiquitination[1]
162LNFKDVLKEKADLGEubiquitination[1]
643DDIHNALKQTPKVLVubiquitination[1, 2]
692GIMSPLAKKKLLSQVubiquitination[1]
693IMSPLAKKKLLSQVSubiquitination[1]
719SPPPLISKKKLIARDubiquitination[1]
774KTINDIFKHEKLSRSubiquitination[1]
777NDIFKHEKLSRSDPHubiquitination[1, 2]
790PHRCSFSKHHLNPLAubiquitination[1]
855LHNEQTSKYPSRDMYubiquitination[3]
878PSHRHQEKLHVNYLTubiquitination[1]
893SLHLQDKKSAAAEAPubiquitination[1, 2]
912PTDLSLPKNPHKPTGubiquitination[1]
916SLPKNPHKPTGKVLGubiquitination[1]
935TTGPQESKGISQFQVubiquitination[1, 2]
967MTMSGPKKYPESLSRubiquitination[1, 2]
1026LDLVIAGKKARAVSPubiquitination[1, 4]
1027DLVIAGKKARAVSPLacetylation[5]
1027DLVIAGKKARAVSPLubiquitination[1, 2]
1038VSPLDPSKEVSGKEKubiquitination[1, 2]
1045KEVSGKEKASEQESEubiquitination[1]
1070GGGSEGHKLPLSSPIubiquitination[1, 2]
1187SSVHPSTKL******ubiquitination[1, 2, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 Transcription coactivator that binds to the 5'-AATA[CT]- 3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2- ARID5B complex acts as a coactivator of HNF4A in liver. Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation. Represses the cytomegalovirus enhancer. 
Sequence Annotation
 DOMAIN 318 410 ARID.
 MOD_RES 336 336 N6,N6-dimethyllysine.  
Keyword
 3D-structure; Activator; Alternative splicing; Complete proteome; DNA-binding; Methylation; Nucleus; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1188 AA 
Protein Sequence
MEPNSLQWVG SPCGLHGPYI FYKAFQFHLE GKPRILSLGD FFFVRCTPKD PICIAELQLL 60
WEERTSRQLL SSSKLYFLPE DTPQGRNSDH GEDEVIAVSE KVIVKLEDLV KWVHSDFSKW 120
RCGFHAGPVK TEALGRNGQK EALLKYRQST LNSGLNFKDV LKEKADLGED EEETNVIVLS 180
YPQYCRYRSM LKRIQDKPSS ILTDQFALAL GGIAVVSRNP QILYCRDTFD HPTLIENESI 240
CDEFAPNLKG RPRKKKPCPQ RRDSFSGVKD SNNNSDGKAV AKVKCEARSA LTKPKNNHNC 300
KKVSNEEKPK VAIGEECRAD EQAFLVALYK YMKERKTPIE RIPYLGFKQI NLWTMFQAAQ 360
KLGGYETITA RRQWKHIYDE LGGNPGSTSA ATCTRRHYER LILPYERFIK GEEDKPLPPI 420
KPRKQENSSQ ENENKTKVSG TKRIKHEIPK SKKEKENAPK PQDAAEVSSE QEKEQETLIS 480
QKSIPEPLPA ADMKKKIEGY QEFSAKPLAS RVDPEKDNET DQGSNSEKVA EEAGEKGPTP 540
PLPSAPLAPE KDSALVPGAS KQPLTSPSAL VDSKQESKLC CFTESPESEP QEASFPSFPT 600
TQPPLANQNE TEDDKLPAMA DYIANCTVKV DQLGSDDIHN ALKQTPKVLV VQSFDMFKDK 660
DLTGPMNENH GLNYTPLLYS RGNPGIMSPL AKKKLLSQVS GASLSSSYPY GSPPPLISKK 720
KLIARDDLCS SLSQTHHGQS TDHMAVSRPS VIQHVQSFRS KPSEERKTIN DIFKHEKLSR 780
SDPHRCSFSK HHLNPLADSY VLKQEIQEGK DKLLEKRALP HSHMPSFLAD FYSSPHLHSL 840
YRHTEHHLHN EQTSKYPSRD MYRESENSSF PSHRHQEKLH VNYLTSLHLQ DKKSAAAEAP 900
TDDQPTDLSL PKNPHKPTGK VLGLAHSTTG PQESKGISQF QVLGSQSRDC HPKACRVSPM 960
TMSGPKKYPE SLSRSGKPHH VRLENFRKME GMVHPILHRK MSPQNIGAAR PIKRSLEDLD 1020
LVIAGKKARA VSPLDPSKEV SGKEKASEQE SEGSKAAHGG HSGGGSEGHK LPLSSPIFPG 1080
LYSGSLCNSG LNSRLPAGYS HSLQYLKNQT VLSPLMQPLA FHSLVMQRGI FTSPTNSQQL 1140
YRHLAAATPV GSSYGDLLHN SIYPLAAINP QAAFPSSQLS SVHPSTKL 1188 
Gene Ontology
 GO:0005634; C:nucleus; IC:GDB.
 GO:0044212; F:transcription regulatory region DNA binding; IDA:UniProtKB.
 GO:0060612; P:adipose tissue development; ISS:UniProtKB.
 GO:0030325; P:adrenal gland development; IEA:Compara.
 GO:0048468; P:cell development; IEA:Compara.
 GO:0060325; P:face morphogenesis; IEA:Compara.
 GO:0045444; P:fat cell differentiation; IEA:Compara.
 GO:0060613; P:fat pad development; IEA:Compara.
 GO:0008585; P:female gonad development; IEA:Compara.
 GO:0010761; P:fibroblast migration; IEA:Compara.
 GO:0001822; P:kidney development; IEA:Compara.
 GO:0001889; P:liver development; TAS:UniProtKB.
 GO:0008584; P:male gonad development; IEA:Compara.
 GO:0035264; P:multicellular organism growth; IEA:Compara.
 GO:0048644; P:muscle organ morphogenesis; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; TAS:GDB.
 GO:0060021; P:palate development; IEA:Compara.
 GO:0048008; P:platelet-derived growth factor receptor signaling pathway; IEA:Compara.
 GO:0051091; P:positive regulation of sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.
 GO:0009791; P:post-embryonic development; IEA:Compara.
 GO:0048705; P:skeletal system morphogenesis; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001606; ARID/BRIGHT_DNA-bd. 
Pfam
 PF01388; ARID 
SMART
 SM00501; BRIGHT 
PROSITE
 PS51011; ARID 
PRINTS