CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037704
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 60S ribosomal protein L8 
Protein Synonyms/Alias
  
Gene Name
 RPL8 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
10RVIRGQRKGAGSVFRubiquitination[1]
42AERHGYIKGIVKDIIubiquitination[1, 2]
46GYIKGIVKDIIHDPGacetylation[2, 3]
46GYIKGIVKDIIHDPGubiquitination[1, 2, 4, 5, 6]
60GRGAPLAKVVFRDPYubiquitination[1, 4, 5, 6, 7]
92GQFVYCGKKAQLNIGubiquitination[7]
93QFVYCGKKAQLNIGNubiquitination[1]
119IVCCLEEKPGDRGKLubiquitination[1, 5, 6, 7]
156AGGGRIDKPILKAGRubiquitination[1, 2, 5, 7]
160RIDKPILKAGRAYHKubiquitination[1, 2, 4, 5, 6, 7]
167KAGRAYHKYKAKRNCubiquitination[5]
213RDAPAGRKVGLIAARubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 235 AA 
Protein Sequence
MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK 60
VVFRDPYRFK KRTELFIAAE GIHTGQFVYC GKKAQLNIGN VLPVGTMPEG TIVCCLEEKP 120
GDRGKLARAS GNYATVISSA NRAVVGVVAG GGRIDKPILK AGRAYHKYKA KRNCWPRVRG 180
VAMNPVEHPF GGGNHQHIGK PSTIRRDAPA GRKVGLIAAR RTGRLRGTKT VQEKE 235 
Gene Ontology
 GO:0005840; C:ribosome; IEA:InterPro.
 GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
 GO:0006412; P:translation; IEA:InterPro. 
Interpro
 IPR012340; NA-bd_OB-fold.
 IPR022666; Rbsml_prot_L2_RNA-bd_dom.
 IPR014722; Rib_L2_dom2.
 IPR002171; Ribosomal_L2.
 IPR022669; Ribosomal_L2_C.
 IPR022671; Ribosomal_L2_CS.
 IPR014726; Ribosomal_L2_dom3.
 IPR008991; Translation_prot_SH3-like. 
Pfam
 PF00181; Ribosomal_L2
 PF03947; Ribosomal_L2_C 
SMART
  
PROSITE
 PS00467; RIBOSOMAL_L2 
PRINTS