CPLM-020203

Genbank Nucleotide ID

Oxidoreductase HTATIP2

Protein Synonyms/Alias

30 kDa HIV-1 TAT-interacting protein; HIV-1 TAT-interactive protein 2

Homo sapiens (Human)

Position	Peptide	Type	References
36	ETGRVLLKEILEQGL	ubiquitination	[1, 2, 3]
98	CLGTTRGKAGAEGFV	ubiquitination	[4]
114	VDRDYVLKSAELAKA	ubiquitination	[4]
137	LSSKGADKSSNFLYL	ubiquitination	[1, 2, 3, 4, 5, 6]
186	PGEWLVRKFFGSLPD	ubiquitination	[2]
220	NVVRPRDKQMELLEN	ubiquitination	[1]
228	QMELLENKAIHDLGK	ubiquitination	[2]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[5] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
[6] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]

Functional Description

Oxidoreductase required for tumor suppression. NAPDH- bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import. Isoform 1 is a metastasis suppressor with proapoptotic as well as antiangiogenic properties. Isoform 2 has an antiapoptotic effect.

NP_BIND 19 52 NADP.
ACT_SITE 143 143 Proton acceptor (Probable).
ACT_SITE 147 147 Probable.
BINDING 131 131 Substrate (Probable).
MOD_RES 45 45 Phosphoserine (By similarity).

3D-structure; Alternative splicing; Angiogenesis; Apoptosis; Complete proteome; Cytoplasm; Developmental protein; Differentiation; Host-virus interaction; NADP; Nucleus; Oxidoreductase; Phosphoprotein; Polymorphism; Reference proteome; Tumor suppressor.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IDA:UniProtKB.
GO:0005635; C:nuclear envelope; IDA:UniProtKB.
GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
GO:0003713; F:transcription coactivator activity; TAS:ProtInc.
GO:0001525; P:angiogenesis; IEA:UniProtKB-KW.
GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
GO:0043066; P:negative regulation of apoptotic process; TAS:UniProtKB.
GO:0051170; P:nuclear import; IDA:UniProtKB.
GO:0045765; P:regulation of angiogenesis; IDA:UniProtKB.
GO:0006357; P:regulation of transcription from RNA polymerase II promoter; TAS:ProtInc.
GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW.

IPR016040; NAD(P)-bd_dom.