UniProt Accession

 SYE_ECOLI; P04805 

Genbank Protein ID

 X63976; M13687; U00096; AP009048; X55737 

Genbank Nucleotide ID

 CAA45391.1; AAA65715.1; AAC75457.1; BAA16272.1; CAA39269.1 

Protein Name

 Glutamate--tRNA ligase 

Protein Synonyms/Alias

 Glutamyl-tRNA synthetase; GluRS 

Gene Name

 gltX 

Gene Synonyms/Alias

 b2400; JW2395 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
102	AYKCYCSKERLEALR	acetylation	[1]
215	PRQINILKALKAPVP	acetylation	[1]
236	MINGDDGKKLSKRHG	acetylation	[1]
237	INGDDGKKLSKRHGA	acetylation	[1]
284	FTREEMIKYFTLNAV	acetylation	[1]
284	FTREEMIKYFTLNAV	pupylation	[2]
345	PQLADLVKLLGERCK	acetylation	[1]
377	EFDADAAKKHLRPVA	acetylation	[1]
378	FDADAAKKHLRPVAR	acetylation	[3]
423	ELEVGMGKVGMPLRV	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Reconstitution of the Mycobacterium tuberculosis pupylation pathway in Escherichia coli.
 Cerda-Maira FA, McAllister F, Bode NJ, Burns KE, Gygi SP, Darwin KH.
 EMBO Rep. 2011 Jul 8;12(8):863-70. [PMID: 21738222]
 [3] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu). 

Sequence Annotation

 MOTIF 9 19 "HIGH" region.
 MOTIF 237 241 "KMSKS" region.
 METAL 98 98 Zinc.
 METAL 100 100 Zinc.
 METAL 125 125 Zinc.
 METAL 127 127 Zinc.
 BINDING 240 240 ATP (By similarity).  

Keyword

 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Metal-binding; Nucleotide-binding; Protein biosynthesis; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 471 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0004818; F:glutamate-tRNA ligase activity; IDA:EcoCyc.
 GO:0000049; F:tRNA binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IDA:EcoCyc.
 GO:0006424; P:glutamyl-tRNA aminoacylation; IMP:EcoCyc. 

Interpro

 IPR008925; aa-tRNA-synth_I_codon-bd.
 IPR020751; aa-tRNA-synth_I_codon-bd_sub2.
 IPR001412; aa-tRNA-synth_I_CS.
 IPR004527; Glu-tRNA-ligase_Ib_bac/mito.
 IPR000924; Glu/Gln-tRNA-synth_Ib.
 IPR020061; Glu/Gln-tRNA-synth_Ib_a-bdl.
 IPR020058; Glu/Gln-tRNA-synth_Ib_cat-dom.
 IPR014729; Rossmann-like_a/b/a_fold. 

Pfam

 PF00749; tRNA-synt_1c 

SMART

  

PROSITE

 PS00178; AA_TRNA_LIGASE_I 

PRINTS

 PR00987; TRNASYNTHGLU.