CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001587
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Acyl-protein thioesterase 2 
Protein Synonyms/Alias
 APT-2; Lysophospholipase II; LPL-II; LysoPLA II 
Gene Name
 LYPLA2 
Gene Synonyms/Alias
 APT2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
53TIRLPHVKYICPHAPubiquitination[1]
93PEDEAGIKKAAENIKubiquitination[2]
100KKAAENIKALIEHEMubiquitination[1, 2]
108ALIEHEMKNGIPANRubiquitination[1, 2, 3, 4]
164QAANGSAKDLAILQCubiquitination[1]
190FGALTAEKLRSVVTPubiquitination[1, 2]
203TPARVQFKTYPGVMHubiquitination[1]
221PQEMAAVKEFLEKLLubiquitination[1]
226AVKEFLEKLLPPV**ubiquitination[1, 2, 4, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 May hydrolyze fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has lysophospholipase activity (By similarity). Deacylates GAP43. 
Sequence Annotation
 ACT_SITE 122 122 Charge relay system (By similarity).
 ACT_SITE 176 176 Charge relay system (By similarity).
 ACT_SITE 210 210 Charge relay system (By similarity).  
Keyword
 Complete proteome; Cytoplasm; Fatty acid metabolism; Hydrolase; Lipid metabolism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 231 AA 
Protein Sequence
MCGNTMSVPL LTDAATVSGA ERETAAVIFL HGLGDTGHSW ADALSTIRLP HVKYICPHAP 60
RIPVTLNMKM VMPSWFDLMG LSPDAPEDEA GIKKAAENIK ALIEHEMKNG IPANRIVLGG 120
FSQGGALSLY TALTCPHPLA GIVALSCWLP LHRAFPQAAN GSAKDLAILQ CHGELDPMVP 180
VRFGALTAEK LRSVVTPARV QFKTYPGVMH SSCPQEMAAV KEFLEKLLPP V 231 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
 GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-KW. 
Interpro
 IPR003140; PLipase/COase/thioEstase. 
Pfam
 PF02230; Abhydrolase_2 
SMART
  
PROSITE
  
PRINTS