CPLM-020713

Genbank Nucleotide ID

Pyruvate dehydrogenase E1 component subunit beta, mitochondrial

Protein Synonyms/Alias

Mus musculus (Mouse)

Position	Peptide	Type	References
68	AQYDGAYKVSRGLWK	acetylation	[1]
184	PWNSEDAKGLIKSAI	ubiquitination	[2]
227	DFLIPIGKAKIERQG	ubiquitination	[2]
258	EAAAVLSKEGIECEV	acetylation	[3]
336	DVPMPYAKVLEDNSV	acetylation	[1, 3, 4, 5]
336	DVPMPYAKVLEDNSV	ubiquitination	[2]
347	DNSVPQVKDIIFAVK	acetylation	[5]
354	KDIIFAVKKTLNI**	acetylation	[1, 3, 5, 6, 7]
354	KDIIFAVKKTLNI**	succinylation	[7]

[1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
[4] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
[5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
[6] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[7] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]

Functional Description

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle (By similarity).

BINDING 89 89 Thiamine pyrophosphate (By similarity).
MOD_RES 67 67 Phosphotyrosine.

Carbohydrate metabolism; Complete proteome; Direct protein sequencing; Glucose metabolism; Mitochondrion; Oxidoreductase; Phosphoprotein; Pyruvate; Reference proteome; Thiamine pyrophosphate; Transit peptide; Tricarboxylic acid cycle.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
GO:0005739; C:mitochondrion; IDA:MGI.
GO:0045254; C:pyruvate dehydrogenase complex; ISS:UniProtKB.
GO:0004739; F:pyruvate dehydrogenase (acetyl-transferring) activity; IEA:EC.
GO:0004738; F:pyruvate dehydrogenase activity; ISS:UniProtKB.
GO:0006086; P:acetyl-CoA biosynthetic process from pyruvate; ISS:UniProtKB.
GO:0006006; P:glucose metabolic process; IEA:UniProtKB-KW.
GO:0006099; P:tricarboxylic acid cycle; ISS:UniProtKB.

IPR027110; PDHB.
IPR009014; Transketo_C/Pyr-ferredox_oxred.
IPR015941; Transketolase-like_C.
IPR005475; Transketolase-like_Pyr-bd.
IPR005476; Transketolase_C.

PF02779; Transket_pyr
PF02780; Transketolase_C

SM00861; Transket_pyr