CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004801
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cytochrome c oxidase subunit 4 isoform 1, mitochondrial 
Protein Synonyms/Alias
 Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1 
Gene Name
 Cox4i1 
Gene Synonyms/Alias
 Cox4; Cox4a 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
29RAHGSVVKSEDYAFPacetylation[1, 2, 3, 4, 5, 6]
29RAHGSVVKSEDYAFPsuccinylation[5]
29RAHGSVVKSEDYAFPubiquitination[7]
60TMLSASQKALKEKEKacetylation[2, 3, 4, 5, 6, 8]
60TMLSASQKALKEKEKsuccinylation[5]
60TMLSASQKALKEKEKubiquitination[7]
65SQKALKEKEKADWSSacetylation[2]
67KALKEKEKADWSSLSacetylation[2]
78SSLSRDEKVQLYRIQacetylation[2]
149TKRMLDMKANPIQGFubiquitination[7]
159PIQGFSAKWDYDKNEacetylation[6]
164SAKWDYDKNEWKK**acetylation[1, 6]
168DYDKNEWKK******acetylation[2]
Reference
 [1] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [6] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [7] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [8] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758
Functional Description
 This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. 
Sequence Annotation
 MOD_RES 60 60 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Complete proteome; Direct protein sequencing; Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 169 AA 
Protein Sequence
MLASRALSLI GKRAISTSVC LRAHGSVVKS EDYAFPTYAD RRDYPLPDVA HVTMLSASQK 60
ALKEKEKADW SSLSRDEKVQ LYRIQFNESF AEMNRGTNEW KTVVGMAMFF IGFTALVLIW 120
EKSYVYGPIP HTFDRDWVAM QTKRMLDMKA NPIQGFSAKW DYDKNEWKK 169 
Gene Ontology
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0004129; F:cytochrome-c oxidase activity; IEA:InterPro. 
Interpro
 IPR013288; Cyt_c_oxidase_su4.
 IPR004203; Cyt_c_oxidase_su4_fam. 
Pfam
 PF02936; COX4 
SMART
  
PROSITE
  
PRINTS
 PR01873; CYTCOXIDASE4.